A subtractive gene expression screen suggests a role for vanin-1 in testis development in mice

Yoshihiko Uehara, Chisato Mori, Tetsuo Noda, Kohei Shiota, Naomi Kitamura

Research output: Contribution to journalArticlepeer-review

56 Citations (Scopus)

Abstract

The molecular pathways leading from indifferent mammalian gonad to either testis or ovary are not well understood. A number of genes, including the Y-linked sex determining gene SRY, have been shown to play roles in sex determination or differentiation, but there are clearly many missing elements to be found. We used suppression-subtractive hybridization to construct normalized cDNA libraries enriched for male-specific or female-specific transcripts in mouse fetal gonads. We describe the strategy used to efficiently screen these libraries for candidate sex-determination and gonado-genesis genes. One gene arising from these screens is vanin-1, which encodes a protein implicated in the induction of cell migration into the thymus. We find that vanin-1 is expressed male-specifically in Sertoli cells of the developing testis and may be involved in inducing cell migration from the adjacent mesonephros, a process known to be critical for testis development. This screening approach is likely to be applicable to the isolation and study of genes involved in a variety of developmental systems. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)124-135
Number of pages12
JournalGenesis
Volume27
Issue number3
Publication statusPublished - 2000 Sep 14

Keywords

  • Gonadal development
  • Sex determination
  • Sex differentiation
  • Suppression PCR
  • Suppression-subtractive hybridization
  • Vanin-1

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

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