A subset of hypersensitive response marker genes, including HSR203J, is the downstream target of a spermine signal transduction pathway in tobacco

Yoshihiro Takahashi, Yukiko Uehara, Thomas Berberich, Akiko Ito, Hiromasa Saitoh, Atsushi Miyazaki, Ryohei Terauchi, Tomonobu Kusano

    Research output: Contribution to journalArticlepeer-review

    104 Citations (Scopus)

    Abstract

    A cellular signal transduction pathway induced by the polyamine, spermine (Spm), and transmitted by mitochondrial dysfunction is proposed in tobacco. In this investigation, we further resolve the pathway by identifying a subset of hypersensitive response (HR) marker genes as downstream components. In a previous report, we identified harpin-induced 1 (HIN1) and two closely related genes as responsive to Spm. Other HR marker genes, HSR203J, HMGR, HSR201, and HSR515, are also Spm-responsive. Induction of these HR marker genes, including HIN1, by Spm was suppressed by pre-treatment with antioxidants, calcium channel blockers, inhibitor of mitochondrial permeability transition pore openings, and blockers of amine oxidase/polyamine oxidase. Such quenching is also observed for Spm-induced activation of two mitogen-activated protein kinases (MAPKs), salicylic acid-induced protein kinase (SIPK), and wound-induced protein kinase (WIPK), and upregulation of the WIPK gene, suggesting that all these components are part of the same signaling pathway. Furthermore, gain-of-function and loss-of-function studies on MAPK cascade members reveal that the expression of Spm-induced HR marker genes varies with respect to involvement of SIPK/WIPK activation.

    Original languageEnglish
    Pages (from-to)586-595
    Number of pages10
    JournalPlant Journal
    Volume40
    Issue number4
    DOIs
    Publication statusPublished - 2004 Nov

    Keywords

    • Hypersensitive response
    • Mitochondria
    • Signal transmitter
    • Spermine
    • Target gene
    • Tobacco

    ASJC Scopus subject areas

    • Genetics
    • Plant Science
    • Cell Biology

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