TY - JOUR
T1 - A subdomain of the endoplasmic reticulum forms a cradle for autophagosome formation
AU - Hayashi-Nishino, Mitsuko
AU - Fujita, Naonobu
AU - Noda, Takeshi
AU - Yamaguchi, Akihito
AU - Yoshimori, Tamotsu
AU - Yamamoto, Akitsugu
N1 - Funding Information:
We thank H. Nishioka and N. Kajimura for technical assistance with electron tomography, K. Nishino for assistance with setting up a computer and for useful comments on figure presentations, H. Omori for technical assistance with immunoelectron microscopy and K. Matsunaga and N. Taguchi for technical assistance with plasmid construction. This study was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
PY - 2009/12
Y1 - 2009/12
N2 - Autophagy is a bulk degradation process in eukaryotic cells and has fundamental roles in cellular homeostasis.The origin and source of autophagosomal membranes are long-standing questions in the field. Using electron microscopy, we show that, in mammalian culture cells, the endoplasmic reticulum (ER) associates with early autophagic structures called isolation membranes (IMs). Overexpression of an Atg4B mutant, which causes defects in autophagosome formation, induces the accumulation of ER-IM complexes. Electron tomography revealed that the ER-IM complex appears as a subdomain of the ER that formed a cradle encircling the IM, and showed that both ER and isolation membranes are interconnected.
AB - Autophagy is a bulk degradation process in eukaryotic cells and has fundamental roles in cellular homeostasis.The origin and source of autophagosomal membranes are long-standing questions in the field. Using electron microscopy, we show that, in mammalian culture cells, the endoplasmic reticulum (ER) associates with early autophagic structures called isolation membranes (IMs). Overexpression of an Atg4B mutant, which causes defects in autophagosome formation, induces the accumulation of ER-IM complexes. Electron tomography revealed that the ER-IM complex appears as a subdomain of the ER that formed a cradle encircling the IM, and showed that both ER and isolation membranes are interconnected.
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U2 - 10.1038/ncb1991
DO - 10.1038/ncb1991
M3 - Article
C2 - 19898463
AN - SCOPUS:71649087199
VL - 11
SP - 1433
EP - 1437
JO - Nature Cell Biology
JF - Nature Cell Biology
SN - 1465-7392
IS - 12
ER -