TY - JOUR
T1 - A strategic sampling design revealed the local genetic structure of cold-water fluvial sculpin
T2 - a focus on groundwater-dependent water temperature heterogeneity
AU - Nakajima, Souta
AU - Sueyoshi, Masanao
AU - Hirota, Shun K.
AU - Ishiyama, Nobuo
AU - Matsuo, Ayumi
AU - Suyama, Yoshihisa
AU - Nakamura, Futoshi
N1 - Funding Information:
We are grateful to Jorge García Molinos for support with the data preparation. We thank the staff of the University of Tokyo Hokkaido Forest for their cooperation in selecting study sites. We also thank Suzuki K., Hotta W., Nishio D., Motosugi N., Kawai H., and Zakoh K. of Hokkaido University for their help in conducting the field sampling and laboratory work. We appreciate anonymous reviewers for their valuable comments to the earlier versions of the manuscript. This study is partly supported by the research fund for the Ishikari and Tokachi Rivers provided by the Ministry of Land, Infrastructure, Transport, and Tourism of Japan.
Publisher Copyright:
© 2021, The Author(s), under exclusive licence to The Genetics Society.
PY - 2021/10
Y1 - 2021/10
N2 - A key piece of information for ecosystem management is the relationship between the environment and population genetic structure. However, it is difficult to clearly quantify the effects of environmental factors on genetic differentiation because of spatial autocorrelation and analytical problems. In this study, we focused on stream ecosystems and the environmental heterogeneity caused by groundwater and constructed a sampling design in which geographic distance and environmental differences are not correlated. Using multiplexed ISSR genotyping by sequencing (MIG-seq) method, a fine-scale population genetics study was conducted in fluvial sculpin Cottus nozawae, for which summer water temperature is the determinant factor in distribution and survival. There was a clear genetic structure in the watershed. Although a significant isolation-by-distance pattern was detected in the watershed, there was no association between genetic differentiation and water temperature. Instead, asymmetric gene flow from relatively low-temperature streams to high-temperature streams was detected, indicating the importance of low-temperature streams and continuous habitats. The groundwater-focused sampling strategy yielded insightful results for conservation.
AB - A key piece of information for ecosystem management is the relationship between the environment and population genetic structure. However, it is difficult to clearly quantify the effects of environmental factors on genetic differentiation because of spatial autocorrelation and analytical problems. In this study, we focused on stream ecosystems and the environmental heterogeneity caused by groundwater and constructed a sampling design in which geographic distance and environmental differences are not correlated. Using multiplexed ISSR genotyping by sequencing (MIG-seq) method, a fine-scale population genetics study was conducted in fluvial sculpin Cottus nozawae, for which summer water temperature is the determinant factor in distribution and survival. There was a clear genetic structure in the watershed. Although a significant isolation-by-distance pattern was detected in the watershed, there was no association between genetic differentiation and water temperature. Instead, asymmetric gene flow from relatively low-temperature streams to high-temperature streams was detected, indicating the importance of low-temperature streams and continuous habitats. The groundwater-focused sampling strategy yielded insightful results for conservation.
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U2 - 10.1038/s41437-021-00468-z
DO - 10.1038/s41437-021-00468-z
M3 - Article
C2 - 34417564
AN - SCOPUS:85113216091
VL - 127
SP - 413
EP - 422
JO - Heredity
JF - Heredity
SN - 0018-067X
IS - 4
ER -