A starvation-specific serine protease gene, isp6+, is involved in both autophagy and sexual development in Schizosaccharomyces pombe

Akio Nakashima, Takahiro Hasegawa, Saori Mori, Masaru Ueno, Shigeyasu Tanaka, Takashi Ushimaru, Shusei Sato, Masahiro Uritani

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Schizosaccharomyces pombe isp6+ gene encodes a vacuolar serine protease, which is specifically induced during nitrogen starvation. An isp6-disruption mutant, isp6Δ, grew normally under normal conditions but was defective in large-scale protein degradation during nitrogen starvation, a hallmark of autophagy. Vacuoles are the organelles for such drastic protein degradation but those of isp6Ddelta; were apparently aberrant. isp6Δ was infertile under nitrogen source-free conditions with poor expression of ste11+, a gene critical for sexual development. A protein kinase A-disruption mutant, pka1Δ, is prone to sexual development because expression of ste11+ is derepressed. However, isp6Δpka1Δ still showed defects in ste11+ expression and sexual development under nitrogen source-free conditions. isp6Δ and isp6Δ pka1Δ were able to initiate sexual development to produce spores when only a small amount of a nitrogen source was present. Pat1 protein kinase negatively controls meiosis, and a temperature-sensitive mutant of pat1, pat1-114, initiates meiosis irrespective of ploidy at the restrictive temperature. However, isp6Δpat1-114 did not start meiosis under nitrogen source-free conditions even at the restrictive temperature. These observations suggest that isp6+ contributes to sexual development by providing a nitrogen source through autophagy.

Original languageEnglish
Pages (from-to)403-413
Number of pages11
JournalCurrent Genetics
Volume49
Issue number6
DOIs
Publication statusPublished - 2006 Jun
Externally publishedYes

Keywords

  • Autophagy
  • Fission yeast
  • Nitrogen starvation
  • Protein degradation
  • Sexual development

ASJC Scopus subject areas

  • Genetics

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