A soluble nonglycosylated recombinant infectious hematopoietic necrosis virus (IHNV) G-protein induces IFNs in rainbow trout (Oncorhynchus mykiss)

Noel Verjan, Ei Lin Ooi, Tomonori Nochi, Hidehiro Kondo, Ikuo Hirono, Takashi Aoki, Hiroshi Kiyono, Yoshikazu Yuki

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Viral glycoproteins interact with cell-surface receptors to mediate virus entry and innate immune system activation. We found that a soluble recombinant infectious hematopoietic necrosis virus G-protein (rIHNV-G) stimulated an early innate immune response mediated by proinflammatory cytokines, IFN1 and IFN-γ in rainbow trout (Oncorhynchus mykiss) fry. Expression of both IFN1 and IFN-γ mRNA transcripts was an early event and was rIHNV-G dose-dependent. In addition, preliminary evidence revealed that the innate immune response induced by rIHNV-G protein could protect rainbow trout fry from a subsequent IHNV virus challenge. Finally, the binding and distribution of FITC-rIHNV-G protein on rainbow trout spleen and head kidney leukocytes resemble morphological changes which occur on the cell membrane during antigen-receptor interaction including membrane reorganization, patching, polarization and capping. Thus a soluble nonglycosylated rIHNV-G protein could mediate the activation of rainbow trout leukocytes, with concomitant production of proinflammatory cytokines and IFNs.

Original languageEnglish
Pages (from-to)170-180
Number of pages11
JournalFish and Shellfish Immunology
Volume25
Issue number1-2
DOIs
Publication statusPublished - 2008 Jul
Externally publishedYes

Keywords

  • IFN
  • IHNV
  • Innate immune response
  • Leukocyte activation
  • Recombinant G protein

ASJC Scopus subject areas

  • Environmental Chemistry
  • Aquatic Science

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