Abstract
A flexible biosensor using a phospholipid polymer to immobilization of glucose oxidase (GOD) was fabricated and tested. At first, an enzyme membrane formed by immobilizing GOD onto a porous polytetrafluoroethylene (PTFE) membrane using the phospholipid polymer (2-methacryloyloxyethyl phosphorylcholine (MPC) copolymerized with 2-ethylhexylmethacrylate (EHMA): PMEH) was evaluated. According to the result of amperometric measurement, average density of GOD to be immobilized was optimized to 38.9 units cm-2. Temperature and pH dependences were also investigated. Then, a flexible glucose sensor was fabricated by immobilizing GOD onto a flexible hydrogen peroxide electrode using PMEH. The flexible glucose sensor showed a linear relationship between output currents and glucose concentration in 0.05-1.00 mmol L-1, with a correlation coefficient of 0.999. The calibration range covered the normal tear glucose level of 0.14-0.23 mmol L-1. This indicates that the flexible biosensor is considered to be useful for monitoring of glucose in tear fluids.
Original language | English |
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Pages (from-to) | 837-842 |
Number of pages | 6 |
Journal | Biomedical microdevices |
Volume | 11 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2009 Apr 14 |
Keywords
- Biosensor
- Flexible
- Glucose sensor
- Phospholipid polymer
ASJC Scopus subject areas
- Biomedical Engineering
- Molecular Biology