A soft and flexible biosensor using a phospholipid polymer for continuous glucose monitoring

Ming Xing Chu; Hiroyuki Kudo; Takayuki Shirai; Kumiko Miyajima; Hirokazu Saito; Nobuyuki Morimoto; Kazuyoshi Yano; Yasuhiko Iwasaki; Kazunari Akiyoshi; Kohji Mitsubayashi

doi:10.1007/s10544-009-9300-1

A soft and flexible biosensor using a phospholipid polymer for continuous glucose monitoring

Ming Xing Chu, Hiroyuki Kudo, Takayuki Shirai, Kumiko Miyajima, Hirokazu Saito, Nobuyuki Morimoto, Kazuyoshi Yano, Yasuhiko Iwasaki, Kazunari Akiyoshi, Kohji Mitsubayashi

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

A flexible biosensor using a phospholipid polymer to immobilization of glucose oxidase (GOD) was fabricated and tested. At first, an enzyme membrane formed by immobilizing GOD onto a porous polytetrafluoroethylene (PTFE) membrane using the phospholipid polymer (2-methacryloyloxyethyl phosphorylcholine (MPC) copolymerized with 2-ethylhexylmethacrylate (EHMA): PMEH) was evaluated. According to the result of amperometric measurement, average density of GOD to be immobilized was optimized to 38.9 units cm^-2. Temperature and pH dependences were also investigated. Then, a flexible glucose sensor was fabricated by immobilizing GOD onto a flexible hydrogen peroxide electrode using PMEH. The flexible glucose sensor showed a linear relationship between output currents and glucose concentration in 0.05-1.00 mmol L^-1, with a correlation coefficient of 0.999. The calibration range covered the normal tear glucose level of 0.14-0.23 mmol L^-1. This indicates that the flexible biosensor is considered to be useful for monitoring of glucose in tear fluids.

Original language	English
Pages (from-to)	837-842
Number of pages	6
Journal	Biomedical microdevices
Volume	11
Issue number	4
DOIs	https://doi.org/10.1007/s10544-009-9300-1
Publication status	Published - 2009
Externally published	Yes

Keywords

Biosensor
Flexible
Glucose sensor
Phospholipid polymer

ASJC Scopus subject areas

Biomedical Engineering
Molecular Biology

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10.1007/s10544-009-9300-1

Cite this

A soft and flexible biosensor using a phospholipid polymer for continuous glucose monitoring. / Chu, Ming Xing; Kudo, Hiroyuki; Shirai, Takayuki; Miyajima, Kumiko; Saito, Hirokazu; Morimoto, Nobuyuki; Yano, Kazuyoshi; Iwasaki, Yasuhiko; Akiyoshi, Kazunari; Mitsubayashi, Kohji.

In: Biomedical microdevices, Vol. 11, No. 4, 2009, p. 837-842.

Research output: Contribution to journal › Article › peer-review

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title = "A soft and flexible biosensor using a phospholipid polymer for continuous glucose monitoring",

abstract = "A flexible biosensor using a phospholipid polymer to immobilization of glucose oxidase (GOD) was fabricated and tested. At first, an enzyme membrane formed by immobilizing GOD onto a porous polytetrafluoroethylene (PTFE) membrane using the phospholipid polymer (2-methacryloyloxyethyl phosphorylcholine (MPC) copolymerized with 2-ethylhexylmethacrylate (EHMA): PMEH) was evaluated. According to the result of amperometric measurement, average density of GOD to be immobilized was optimized to 38.9 units cm-2. Temperature and pH dependences were also investigated. Then, a flexible glucose sensor was fabricated by immobilizing GOD onto a flexible hydrogen peroxide electrode using PMEH. The flexible glucose sensor showed a linear relationship between output currents and glucose concentration in 0.05-1.00 mmol L-1, with a correlation coefficient of 0.999. The calibration range covered the normal tear glucose level of 0.14-0.23 mmol L-1. This indicates that the flexible biosensor is considered to be useful for monitoring of glucose in tear fluids.",

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author = "Chu, {Ming Xing} and Hiroyuki Kudo and Takayuki Shirai and Kumiko Miyajima and Hirokazu Saito and Nobuyuki Morimoto and Kazuyoshi Yano and Yasuhiko Iwasaki and Kazunari Akiyoshi and Kohji Mitsubayashi",

note = "Funding Information: Acknowledgements This work was partly supported by Japan Society for the promotion of Science (JSPS) Grant-in-Aid for Scientific Research System and by MEXT (Ministry of Education, Culture, Sports, Science and Technology) Special Funds for Education and Research “Advanced Reasearch Program in Sensing Biology”.",

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AU - Chu, Ming Xing

AU - Kudo, Hiroyuki

AU - Shirai, Takayuki

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AU - Saito, Hirokazu

AU - Morimoto, Nobuyuki

AU - Yano, Kazuyoshi

AU - Iwasaki, Yasuhiko

AU - Akiyoshi, Kazunari

AU - Mitsubayashi, Kohji

N1 - Funding Information: Acknowledgements This work was partly supported by Japan Society for the promotion of Science (JSPS) Grant-in-Aid for Scientific Research System and by MEXT (Ministry of Education, Culture, Sports, Science and Technology) Special Funds for Education and Research “Advanced Reasearch Program in Sensing Biology”.

PY - 2009

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N2 - A flexible biosensor using a phospholipid polymer to immobilization of glucose oxidase (GOD) was fabricated and tested. At first, an enzyme membrane formed by immobilizing GOD onto a porous polytetrafluoroethylene (PTFE) membrane using the phospholipid polymer (2-methacryloyloxyethyl phosphorylcholine (MPC) copolymerized with 2-ethylhexylmethacrylate (EHMA): PMEH) was evaluated. According to the result of amperometric measurement, average density of GOD to be immobilized was optimized to 38.9 units cm-2. Temperature and pH dependences were also investigated. Then, a flexible glucose sensor was fabricated by immobilizing GOD onto a flexible hydrogen peroxide electrode using PMEH. The flexible glucose sensor showed a linear relationship between output currents and glucose concentration in 0.05-1.00 mmol L-1, with a correlation coefficient of 0.999. The calibration range covered the normal tear glucose level of 0.14-0.23 mmol L-1. This indicates that the flexible biosensor is considered to be useful for monitoring of glucose in tear fluids.

AB - A flexible biosensor using a phospholipid polymer to immobilization of glucose oxidase (GOD) was fabricated and tested. At first, an enzyme membrane formed by immobilizing GOD onto a porous polytetrafluoroethylene (PTFE) membrane using the phospholipid polymer (2-methacryloyloxyethyl phosphorylcholine (MPC) copolymerized with 2-ethylhexylmethacrylate (EHMA): PMEH) was evaluated. According to the result of amperometric measurement, average density of GOD to be immobilized was optimized to 38.9 units cm-2. Temperature and pH dependences were also investigated. Then, a flexible glucose sensor was fabricated by immobilizing GOD onto a flexible hydrogen peroxide electrode using PMEH. The flexible glucose sensor showed a linear relationship between output currents and glucose concentration in 0.05-1.00 mmol L-1, with a correlation coefficient of 0.999. The calibration range covered the normal tear glucose level of 0.14-0.23 mmol L-1. This indicates that the flexible biosensor is considered to be useful for monitoring of glucose in tear fluids.

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A soft and flexible biosensor using a phospholipid polymer for continuous glucose monitoring

Abstract

Keywords

ASJC Scopus subject areas

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