A soft and flexible biosensor using a phospholipid polymer for continuous glucose monitoring

Ming Xing Chu, Hiroyuki Kudo, Takayuki Shirai, Kumiko Miyajima, Hirokazu Saito, Nobuyuki Morimoto, Kazuyoshi Yano, Yasuhiko Iwasaki, Kazunari Akiyoshi, Kohji Mitsubayashi

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

A flexible biosensor using a phospholipid polymer to immobilization of glucose oxidase (GOD) was fabricated and tested. At first, an enzyme membrane formed by immobilizing GOD onto a porous polytetrafluoroethylene (PTFE) membrane using the phospholipid polymer (2-methacryloyloxyethyl phosphorylcholine (MPC) copolymerized with 2-ethylhexylmethacrylate (EHMA): PMEH) was evaluated. According to the result of amperometric measurement, average density of GOD to be immobilized was optimized to 38.9 units cm-2. Temperature and pH dependences were also investigated. Then, a flexible glucose sensor was fabricated by immobilizing GOD onto a flexible hydrogen peroxide electrode using PMEH. The flexible glucose sensor showed a linear relationship between output currents and glucose concentration in 0.05-1.00 mmol L-1, with a correlation coefficient of 0.999. The calibration range covered the normal tear glucose level of 0.14-0.23 mmol L-1. This indicates that the flexible biosensor is considered to be useful for monitoring of glucose in tear fluids.

Original languageEnglish
Pages (from-to)837-842
Number of pages6
JournalBiomedical microdevices
Volume11
Issue number4
DOIs
Publication statusPublished - 2009 Apr 14

Keywords

  • Biosensor
  • Flexible
  • Glucose sensor
  • Phospholipid polymer

ASJC Scopus subject areas

  • Biomedical Engineering
  • Molecular Biology

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