A small decrease in rubisco content by individual suppression of RBCS genes leads to improvement of photosynthesis and greater biomass production in rice under conditions of elevated CO2

Keiichi Kanno, Yuji Suzuki, Amane Makino

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Rubisco limits photosynthesis at low CO2 concentrations ([CO2]), but does not limit it at elevated [CO2]. This means that the amount of Rubisco is excessive for photosynthesis at elevated [CO2]. Therefore, we examined whether a small decrease in Rubisco content by individual suppression of the RBCS multigene family leads to increases in photosynthesis and biomass production at elevated [CO2] in rice (Oryza sativa L.). Our previous studies indicated that the individual suppression of RBCS decreased Rubisco content in rice by 10-25%. Three lines of BC2F2 progeny were selected from transgenic plants with individual suppression of OsRBCS2, 3 and 5. Rubisco content in the selected lines was 71-90% that of wild-type plants. These three transgenic lines showed lower rates of CO2 assimilation at low [CO2] (28 Pa) but higher rates of CO2 assimilation at elevated [CO2] (120 Pa). Similarly, the biomass production and relative growth rate (RGR) of the two lines were also smaller at low [CO2] but greater than that of wild-type plants at elevated [CO2]. This greater RGR was caused by the higher net assimilation rate (NAR). When the nitrogen use efficiency (NUE) for the NAR was estimated by dividing the NAR by whole-plant leaf N content, the NUE for NAR at elevated [CO2] was higher in these two lines. Thus, a small decrease in Rubisco content leads to improvements of photosynthesis and greater biomass production in rice under conditions of elevated CO2.

Original languageEnglish
Pages (from-to)635-642
Number of pages8
JournalPlant and Cell Physiology
Volume58
Issue number3
DOIs
Publication statusPublished - 2017 Mar 1

Keywords

  • Biomass
  • Elevated [CO]
  • Oryza sativa
  • Photosynthesis
  • RBCS
  • Rubisco

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

Fingerprint Dive into the research topics of 'A small decrease in rubisco content by individual suppression of RBCS genes leads to improvement of photosynthesis and greater biomass production in rice under conditions of elevated CO<sub>2</sub>'. Together they form a unique fingerprint.

Cite this