TY - JOUR
T1 - A simple method for whole‐cell preparation in electron microscopy
AU - Aizu, Seiei
AU - Itoh, Tsunetoshi
AU - Yamamoto, Toshi Yuki
PY - 1981/11
Y1 - 1981/11
N2 - A simple method for whole‐cell preparation without using gold or platinum grids as substrata for culture is described. Cells were cultured on formvar film over round pores, each 3 mm in diameter, of Thermonox coverslips. The cells on the formvar coated coverslip are fixed, stained, dehydrated in situ, and introduced into a critical point drying apparatus. A small quantity of 0.2% mesh‐cement is applied to slot grids, and they are laid onto the formvar film over the pores of the coverslip. After the grids are removed from the plastic substratum, they are ready for observation under the electron microscope. 1981 Blackwell Science Ltd
AB - A simple method for whole‐cell preparation without using gold or platinum grids as substrata for culture is described. Cells were cultured on formvar film over round pores, each 3 mm in diameter, of Thermonox coverslips. The cells on the formvar coated coverslip are fixed, stained, dehydrated in situ, and introduced into a critical point drying apparatus. A small quantity of 0.2% mesh‐cement is applied to slot grids, and they are laid onto the formvar film over the pores of the coverslip. After the grids are removed from the plastic substratum, they are ready for observation under the electron microscope. 1981 Blackwell Science Ltd
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U2 - 10.1111/j.1365-2818.1981.tb00312.x
DO - 10.1111/j.1365-2818.1981.tb00312.x
M3 - Article
C2 - 7321031
AN - SCOPUS:0019842205
VL - 124
SP - 183
EP - 187
JO - Journal of Microscopy
JF - Journal of Microscopy
SN - 0022-2720
IS - 2
ER -