A simple fed-batch method for transcription and insect cell-free translation

Yoko Sato; Keishi Aizawa; Toru Ezure; Eiji Ando; Nobuyuki Uozumi

doi:10.1016/j.jbiosc.2012.06.015

A simple fed-batch method for transcription and insect cell-free translation

Yoko Sato, Keishi Aizawa, Toru Ezure, Eiji Ando, Nobuyuki Uozumi

ENG - Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

We tested a one batch reaction method for the transcription and the insect cell-free translation from undigested plasmids without any centrifugation steps. The efficiency of protein synthesis reached 74-112% of that achieved using the conventional procedure. This simplified method will help expedite the high throughput insect cell-free protein production.

Original language	English
Pages (from-to)	677-679
Number of pages	3
Journal	Journal of Bioscience and Bioengineering
Volume	114
Issue number	6
DOIs	https://doi.org/10.1016/j.jbiosc.2012.06.015
Publication status	Published - 2012 Dec

Keywords

Eukaryotic proteins
High-throughput
Insect cell-free system
One batch reaction
Undigested plasmids

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

Access to Document

10.1016/j.jbiosc.2012.06.015

Cite this

@article{93b4fad9da0749efb600d968f06f6d8e,

title = "A simple fed-batch method for transcription and insect cell-free translation",

abstract = "We tested a one batch reaction method for the transcription and the insect cell-free translation from undigested plasmids without any centrifugation steps. The efficiency of protein synthesis reached 74-112% of that achieved using the conventional procedure. This simplified method will help expedite the high throughput insect cell-free protein production.",

keywords = "Eukaryotic proteins, High-throughput, Insect cell-free system, One batch reaction, Undigested plasmids",

author = "Yoko Sato and Keishi Aizawa and Toru Ezure and Eiji Ando and Nobuyuki Uozumi",

note = "Funding Information: This work was supported by Grants-in-Aid for Scientific Research ( 22020002 and 22380056) from MEXT and JSPS , and by JST program ( AS2314135E ).",

year = "2012",

month = dec,

doi = "10.1016/j.jbiosc.2012.06.015",

language = "English",

volume = "114",

pages = "677--679",

journal = "Journal of Bioscience and Bioengineering",

issn = "1389-1723",

publisher = "Elsevier",

number = "6",

}

TY - JOUR

T1 - A simple fed-batch method for transcription and insect cell-free translation

AU - Sato, Yoko

AU - Aizawa, Keishi

AU - Ezure, Toru

AU - Ando, Eiji

AU - Uozumi, Nobuyuki

N1 - Funding Information: This work was supported by Grants-in-Aid for Scientific Research ( 22020002 and 22380056) from MEXT and JSPS , and by JST program ( AS2314135E ).

PY - 2012/12

Y1 - 2012/12

N2 - We tested a one batch reaction method for the transcription and the insect cell-free translation from undigested plasmids without any centrifugation steps. The efficiency of protein synthesis reached 74-112% of that achieved using the conventional procedure. This simplified method will help expedite the high throughput insect cell-free protein production.

AB - We tested a one batch reaction method for the transcription and the insect cell-free translation from undigested plasmids without any centrifugation steps. The efficiency of protein synthesis reached 74-112% of that achieved using the conventional procedure. This simplified method will help expedite the high throughput insect cell-free protein production.

KW - Eukaryotic proteins

KW - High-throughput

KW - Insect cell-free system

KW - One batch reaction

KW - Undigested plasmids

UR - http://www.scopus.com/inward/record.url?scp=84868115336&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84868115336&partnerID=8YFLogxK

U2 - 10.1016/j.jbiosc.2012.06.015

DO - 10.1016/j.jbiosc.2012.06.015

M3 - Article

C2 - 22841867

AN - SCOPUS:84868115336

VL - 114

SP - 677

EP - 679

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

SN - 1389-1723

IS - 6

ER -

A simple fed-batch method for transcription and insect cell-free translation

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this