A simple fed-batch method for transcription and insect cell-free translation

Yoko Sato; Keishi Aizawa; Toru Ezure; Eiji Ando; Nobuyuki Uozumi

doi:10.1016/j.jbiosc.2012.06.015

A simple fed-batch method for transcription and insect cell-free translation

Yoko Sato, Keishi Aizawa, Toru Ezure, Eiji Ando, Nobuyuki Uozumi

ENG - Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

We tested a one batch reaction method for the transcription and the insect cell-free translation from undigested plasmids without any centrifugation steps. The efficiency of protein synthesis reached 74-112% of that achieved using the conventional procedure. This simplified method will help expedite the high throughput insect cell-free protein production.

Original language	English
Pages (from-to)	677-679
Number of pages	3
Journal	Journal of Bioscience and Bioengineering
Volume	114
Issue number	6
DOIs	https://doi.org/10.1016/j.jbiosc.2012.06.015
Publication status	Published - 2012 Dec 1

Keywords

Eukaryotic proteins
High-throughput
Insect cell-free system
One batch reaction
Undigested plasmids

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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AU - Sato, Yoko

AU - Aizawa, Keishi

AU - Ezure, Toru

AU - Ando, Eiji

AU - Uozumi, Nobuyuki

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N2 - We tested a one batch reaction method for the transcription and the insect cell-free translation from undigested plasmids without any centrifugation steps. The efficiency of protein synthesis reached 74-112% of that achieved using the conventional procedure. This simplified method will help expedite the high throughput insect cell-free protein production.

AB - We tested a one batch reaction method for the transcription and the insect cell-free translation from undigested plasmids without any centrifugation steps. The efficiency of protein synthesis reached 74-112% of that achieved using the conventional procedure. This simplified method will help expedite the high throughput insect cell-free protein production.

KW - Eukaryotic proteins

KW - High-throughput

KW - Insect cell-free system

KW - One batch reaction

KW - Undigested plasmids

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