Abstract
We tested a one batch reaction method for the transcription and the insect cell-free translation from undigested plasmids without any centrifugation steps. The efficiency of protein synthesis reached 74-112% of that achieved using the conventional procedure. This simplified method will help expedite the high throughput insect cell-free protein production.
Original language | English |
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Pages (from-to) | 677-679 |
Number of pages | 3 |
Journal | Journal of Bioscience and Bioengineering |
Volume | 114 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2012 Dec |
Keywords
- Eukaryotic proteins
- High-throughput
- Insect cell-free system
- One batch reaction
- Undigested plasmids
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology