A simple fed-batch method for transcription and insect cell-free translation

Yoko Sato, Keishi Aizawa, Toru Ezure, Eiji Ando, Nobuyuki Uozumi

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

We tested a one batch reaction method for the transcription and the insect cell-free translation from undigested plasmids without any centrifugation steps. The efficiency of protein synthesis reached 74-112% of that achieved using the conventional procedure. This simplified method will help expedite the high throughput insect cell-free protein production.

Original languageEnglish
Pages (from-to)677-679
Number of pages3
JournalJournal of Bioscience and Bioengineering
Volume114
Issue number6
DOIs
Publication statusPublished - 2012 Dec

Keywords

  • Eukaryotic proteins
  • High-throughput
  • Insect cell-free system
  • One batch reaction
  • Undigested plasmids

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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