A simple and rapid ultra-high-performance liquid chromatography–tandem mass spectrometry method to determine plasma biotin in hemodialysis patients

Shigeaki Yagi, Manabu Nishizawa, Itiro Ando, Shiro Oguma, Emiko Sato, Yutaka Imai, Masako Fujiwara

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

A simple, rapid, and selective method for determination of plasma biotin was developed using ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS). After single-step protein precipitation with methanol, biotin and stable isotope-labeled biotin as an internal standard (IS) were chromatographed on a pentafluorophenyl stationary-phase column (2.1 × 100 mm, 2.7 μm) under isocratic conditions using 10 mm ammonium formate–acetonitrile (93:7, v/v) at a flow rate of 0.6 mL/min. The total chromatographic runtime was 5 min for each injection. Detection was performed in a positive electrospray ionization mode by monitoring selected ion transitions at m/z 245.1/227.0 and 249.1/231.0 for biotin and the IS, respectively. The calibration curve was linear in the range of 0.05–2 ng/mL using 300 μL of plasma. The intra- and inter-day precisions were all <7.1%. The accuracy varied from −0.7 to 8.2%. The developed UHPLC–MS/MS method was successfully applied to determine plasma biotin concentrations in hemodialysis patients.

Original languageEnglish
Pages (from-to)1285-1290
Number of pages6
JournalBiomedical Chromatography
Volume30
Issue number8
DOIs
Publication statusPublished - 2016 Aug 1

Keywords

  • UHPLC–MS/MS
  • biotin
  • hemodialysis
  • human plasma

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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