A simple and rapid method for the analysis of fish histamine by paper electrophoresis

Minoru Sato, Zhi Hua Tao, Kazuhiro Shiozaki, Toshiki Nakano, Toshiyasu Yamaguchi, Takehiko Yokoyama, Nobuhiro Kan-No, Eizoh Nagahisa

    Research output: Contribution to journalArticlepeer-review

    21 Citations (Scopus)


    The described analytical method for histamine determination in fish and seafood consists of sample extraction, adsorption onto a paper disc, application of the paper disc onto electrophoresis paper, electrophoresis for only 10 min, drying, and color developing by Pauly's reagent. Histamine can be satisfactorily detected and completely separated from histidine, carnosine and other Pauly reagent-positive compounds. This method does not require expensive instrumentation and any tedious pretreatment to eliminate potential interference by other imidazole compounds, such as histidine or carnosine. This method can be used to detect histamine in multiple fish and seafood samples simultaneously that contain as little as 15 p.p.m. histamine (1.5 mg/100 g).

    Original languageEnglish
    Pages (from-to)889-892
    Number of pages4
    JournalFisheries Science
    Issue number4
    Publication statusPublished - 2006 Aug


    • Determination
    • Fish
    • Histamine
    • Paper electrophoresis
    • Pauly reagent

    ASJC Scopus subject areas

    • Aquatic Science


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