A simple and rapid method for preparation of 203hg-labeled methylmercury from 203hgcl2 and methylco-balamin

Akira Naganuma, Tsutomu Urano, Nobumasa Imura

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

A rapid and simple method for preparation of 203Hg-labeled methylmercuric chloride (CH3203HgCl) from 203HgCl2 and methylcobalamin is described. More than 99% of 203Hg was methylated with methylcobalaminin 0.01 N HC1 during 1 h. CH3203HgCl formed during the reaction was rapidly and completely separated from cobalamins ana unreacted 203HgCl2 by CM-Sephadex C-25 minicolumn. The low cost procedure for preparation of CH3203HgCl can be completed within 2 h and yields an inorganic 203Hg-free CH3203HgCl which is useful in methylmercury toxicology.

Original languageEnglish
Pages (from-to)69-72
Number of pages4
Journaljournal of pharmacobio-dynamics
Volume8
Issue number1
DOIs
Publication statusPublished - 1985 Jan 1

Keywords

  • 203Hg-labeled methylmercury
  • isolation
  • methylation
  • methylcobalamin
  • synthesis

ASJC Scopus subject areas

  • Pharmacology

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