Abstract
A rapid and simple method for preparation of 203Hg-labeled methylmercuric chloride (CH3203HgCl) from 203HgCl2 and methylcobalamin is described. More than 99% of 203Hg was methylated with methylcobalaminin 0.01 N HC1 during 1 h. CH3203HgCl formed during the reaction was rapidly and completely separated from cobalamins ana unreacted 203HgCl2 by CM-Sephadex C-25 minicolumn. The low cost procedure for preparation of CH3203HgCl can be completed within 2 h and yields an inorganic 203Hg-free CH3203HgCl which is useful in methylmercury toxicology.
| Original language | English |
|---|---|
| Pages (from-to) | 69-72 |
| Number of pages | 4 |
| Journal | journal of pharmacobio-dynamics |
| Volume | 8 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1985 Jan 1 |
Keywords
- 203Hg-labeled methylmercury
- isolation
- methylation
- methylcobalamin
- synthesis
ASJC Scopus subject areas
- Pharmacology