A sequence-ready contig map of the top arm of Arabidopsis thaliana chromosome 3

Shusei Sato, Takakazu Kaneko, Hirokazu Kotani, Reiko Hayashi, Yao Guang Liu, Daisuke Shibata, Satoshi Tabata

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

A fine physical map of the top arm of Arabidopsis thaliana chromosome 3 has been constructed by ordering PI, TAC and BAC clones using the sequences of a variety of DNA markers and end-sequences of clones. The marker sequences used in this study were derived from 58 DNA markers, 93 YAC end-sequences, and 807 end-sequences of P1, TAC and BAC clones. The entire top arm of chromosome 3, except for the centromeric and telomeric regions, was covered by a single contig 13.3 Mb long. This fine physical map will facilitate gene isolation by map-based cloning experiments as well as genome sequencing of the top arm of chromosome 3. The map and end-sequence information are available on the web site KAOS (Kazusa Arabidopsis data Opening Site) at [http://www.kazusa.or.jp/arabi/].

Original languageEnglish
Pages (from-to)117-121
Number of pages5
JournalDNA Research
Volume6
Issue number2
DOIs
Publication statusPublished - 1999

Keywords

  • Arabidopsis thaliana
  • Chromosome 3
  • Physical map
  • Sequence-ready contig map

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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