A sensitive and specific Raman probe based on bisarylbutadiyne for live cell imaging of mitochondria

Hiroyuki Yamakoshi, Almar Palonpon, Kosuke Dodo, Jun Ando, Satoshi Kawata, Katsumasa Fujita, Mikiko Sodeoka

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

We previously showed that bisarylbutadiyne (BADY), which has a conjugated diyne structure, exhibits an intense peak in the cellular Raman-silent region. Here, we synthesized a mitochondria-selective Raman probe by linking bisphenylbutadiyne with triphenylphosphonium, a well-known mitochondrial targeting moiety. This probe, named MitoBADY, has a Raman peak 27 times more intense than that of 5-ethynyl-2′-deoxyuridine. Raman microscopy using submicromolar extracellular probe concentrations successfully visualized mitochondria in living cells. A full Raman spectrum is acquired at each pixel of the scanned sample, and we showed that simultaneous Raman imaging of MitoBADY and endogenous cellular biomolecules can be achieved in a single scan. MitoBADY should be useful for the study of mitochondrial dynamics.

Original languageEnglish
Pages (from-to)664-667
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume25
Issue number3
DOIs
Publication statusPublished - 2015 Feb 1
Externally publishedYes

Keywords

  • Imaging
  • Live cell
  • Mitochondria
  • Raman
  • Triphenylphosphonium

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

Fingerprint

Dive into the research topics of 'A sensitive and specific Raman probe based on bisarylbutadiyne for live cell imaging of mitochondria'. Together they form a unique fingerprint.

Cite this