TY - JOUR
T1 - A sensitive and specific Raman probe based on bisarylbutadiyne for live cell imaging of mitochondria
AU - Yamakoshi, Hiroyuki
AU - Palonpon, Almar
AU - Dodo, Kosuke
AU - Ando, Jun
AU - Kawata, Satoshi
AU - Fujita, Katsumasa
AU - Sodeoka, Mikiko
N1 - Funding Information:
The authors thank Dr. Masaya Okada for technical support and helpful discussions. This work was partly supported by a Grant-in-Aid for Young Scientist (B) ( 23710276 to H.Y. and 2410267 to J.A.) from the Ministry of Education, Culture, Sports, Science, and Technology , Japan.
Publisher Copyright:
© 2014 Elsevier Ltd. All rights reserved.
PY - 2015/2/1
Y1 - 2015/2/1
N2 - We previously showed that bisarylbutadiyne (BADY), which has a conjugated diyne structure, exhibits an intense peak in the cellular Raman-silent region. Here, we synthesized a mitochondria-selective Raman probe by linking bisphenylbutadiyne with triphenylphosphonium, a well-known mitochondrial targeting moiety. This probe, named MitoBADY, has a Raman peak 27 times more intense than that of 5-ethynyl-2′-deoxyuridine. Raman microscopy using submicromolar extracellular probe concentrations successfully visualized mitochondria in living cells. A full Raman spectrum is acquired at each pixel of the scanned sample, and we showed that simultaneous Raman imaging of MitoBADY and endogenous cellular biomolecules can be achieved in a single scan. MitoBADY should be useful for the study of mitochondrial dynamics.
AB - We previously showed that bisarylbutadiyne (BADY), which has a conjugated diyne structure, exhibits an intense peak in the cellular Raman-silent region. Here, we synthesized a mitochondria-selective Raman probe by linking bisphenylbutadiyne with triphenylphosphonium, a well-known mitochondrial targeting moiety. This probe, named MitoBADY, has a Raman peak 27 times more intense than that of 5-ethynyl-2′-deoxyuridine. Raman microscopy using submicromolar extracellular probe concentrations successfully visualized mitochondria in living cells. A full Raman spectrum is acquired at each pixel of the scanned sample, and we showed that simultaneous Raman imaging of MitoBADY and endogenous cellular biomolecules can be achieved in a single scan. MitoBADY should be useful for the study of mitochondrial dynamics.
KW - Imaging
KW - Live cell
KW - Mitochondria
KW - Raman
KW - Triphenylphosphonium
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U2 - 10.1016/j.bmcl.2014.11.080
DO - 10.1016/j.bmcl.2014.11.080
M3 - Article
C2 - 25522818
AN - SCOPUS:84920718973
VL - 25
SP - 664
EP - 667
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
SN - 0960-894X
IS - 3
ER -