TY - JOUR
T1 - A sensitive and specific ELISA for plasma pentosidine
AU - Izuhara, Yuko
AU - Miyata, Toshio
AU - Ueda, Yasuhiko
AU - Suzuki, Daisuke
AU - Asahi, Koichi
AU - Inagi, Reiko
AU - Sakai, Hideto
AU - Kurokawa, Kiyoshi
N1 - Funding Information:
Acknowledgements. We thank Drs Satoshi Sugiyama, Ryouji Tanabe, and Makoto Nishina for providing us plasma of HD and CAPD patients. This study was supported by a grant from Research for the Future Programme of the Japan Society for the Promotion of Science (96L00303).
PY - 1999
Y1 - 1999
N2 - Background. Advanced glycation end products are formed by non-enzymatic glycation and oxidation reaction. Pentosidine is a well-known and characterized structure among them, and has been implicated in the pathogenesis of complications associated with chronic renal failure and long-term dialysis, such as dialysis-related amyloidosis and atherosclerosis. Methods. We established a highly sensitive and specific competitive enzyme-linked immunosorbent assay (ELISA) for plasma pentosidine and applied it to large numbers of plasma samples including haemodialysis (HD) and continuous ambulatory peritoneal dialysis (CAPD) patients. We compared their plasma pentosidine levels determined by the competitive ELISA with those determined by high-performance liquid chromatographic (HPLC) assay currently used. Results. The plasma pentosidine levels determined by the ELISA were correlated well with those determined by sophisticated instrumental HPLC assay, both in non-diabetic and diabetic dialysis patients. Both analyses yielded comparable results, with over 8-fold higher plasma pentosidine levels in HD and CAPD patients, irrespective of the presence or absence of diabetes, as compared to normal subjects and non-uraemic diabetic patients. Conclusions. The competitive ELISA will provide a rapid and convenient determination of plasma pentosidine content and thus be useful to assess the carbonyl stress in uraemic patients.
AB - Background. Advanced glycation end products are formed by non-enzymatic glycation and oxidation reaction. Pentosidine is a well-known and characterized structure among them, and has been implicated in the pathogenesis of complications associated with chronic renal failure and long-term dialysis, such as dialysis-related amyloidosis and atherosclerosis. Methods. We established a highly sensitive and specific competitive enzyme-linked immunosorbent assay (ELISA) for plasma pentosidine and applied it to large numbers of plasma samples including haemodialysis (HD) and continuous ambulatory peritoneal dialysis (CAPD) patients. We compared their plasma pentosidine levels determined by the competitive ELISA with those determined by high-performance liquid chromatographic (HPLC) assay currently used. Results. The plasma pentosidine levels determined by the ELISA were correlated well with those determined by sophisticated instrumental HPLC assay, both in non-diabetic and diabetic dialysis patients. Both analyses yielded comparable results, with over 8-fold higher plasma pentosidine levels in HD and CAPD patients, irrespective of the presence or absence of diabetes, as compared to normal subjects and non-uraemic diabetic patients. Conclusions. The competitive ELISA will provide a rapid and convenient determination of plasma pentosidine content and thus be useful to assess the carbonyl stress in uraemic patients.
KW - Advanced glycation end product
KW - Competitive ELISA
KW - Diabetes
KW - Pentosidine
KW - Uraemia
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U2 - 10.1093/ndt/14.3.576
DO - 10.1093/ndt/14.3.576
M3 - Article
C2 - 10193802
AN - SCOPUS:0032968406
VL - 14
SP - 576
EP - 580
JO - Proceedings of the European Dialysis and Transplant Association - European Renal Association. European Dialysis and Transplant Association - European Renal Association. Congress
JF - Proceedings of the European Dialysis and Transplant Association - European Renal Association. European Dialysis and Transplant Association - European Renal Association. Congress
SN - 0931-0509
IS - 3
ER -
