TY - JOUR
T1 - A screening method for DNA aptamers that bind to a specific, unidentified protein in tissue samples
AU - Noma, Takahisa
AU - Ikebukuro, Kazunori
AU - Sode, Koji
AU - Ohkubo, Takuya
AU - Sakasegawa, Yuji
AU - Hachiya, Naomi
AU - Kaneko, Kiyotoshi
N1 - Funding Information:
Acknowledgement This work is partly supported by the Grant-in-Aid for the 21st Century COE ‘‘Future Nano-Materials’’ from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan.
PY - 2006/9
Y1 - 2006/9
N2 - Aptamers are oligonucleotide ligands with a high affinity to, and specificity for, various target molecules and they are expected to be powerful tools for proteomic analysis. To select aptamers that bind to a specific unidentified protein in tissues for protein analysis, a screening method was developed using chicken skeletal muscle as a model. Target proteins in the target mixture were separated by electrophoresis and transferred to a membrane, and a DNA library was added onto it. The aptamers that bound to the target protein were visualized by chemiluminescence and collected by cutting out the visualized band. The specific aptamers to the target protein were selected by only one round of selection using this screening, suggesting this screening method might be useful for selecting aptamers for proteome analysis.
AB - Aptamers are oligonucleotide ligands with a high affinity to, and specificity for, various target molecules and they are expected to be powerful tools for proteomic analysis. To select aptamers that bind to a specific unidentified protein in tissues for protein analysis, a screening method was developed using chicken skeletal muscle as a model. Target proteins in the target mixture were separated by electrophoresis and transferred to a membrane, and a DNA library was added onto it. The aptamers that bound to the target protein were visualized by chemiluminescence and collected by cutting out the visualized band. The specific aptamers to the target protein were selected by only one round of selection using this screening, suggesting this screening method might be useful for selecting aptamers for proteome analysis.
KW - Aptamer
KW - Electrophoresis blotting
KW - Multiple target proteins
KW - Systematic evolution of ligands by exponential enrichment
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U2 - 10.1007/s10529-006-9106-7
DO - 10.1007/s10529-006-9106-7
M3 - Article
C2 - 16819584
AN - SCOPUS:33747367017
VL - 28
SP - 1377
EP - 1381
JO - Biotechnology Letters
JF - Biotechnology Letters
SN - 0141-5492
IS - 17
ER -