A screening method for DNA aptamers that bind to a specific, unidentified protein in tissue samples

Takahisa Noma, Kazunori Ikebukuro, Koji Sode, Takuya Ohkubo, Yuji Sakasegawa, Naomi Hachiya, Kiyotoshi Kaneko

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Aptamers are oligonucleotide ligands with a high affinity to, and specificity for, various target molecules and they are expected to be powerful tools for proteomic analysis. To select aptamers that bind to a specific unidentified protein in tissues for protein analysis, a screening method was developed using chicken skeletal muscle as a model. Target proteins in the target mixture were separated by electrophoresis and transferred to a membrane, and a DNA library was added onto it. The aptamers that bound to the target protein were visualized by chemiluminescence and collected by cutting out the visualized band. The specific aptamers to the target protein were selected by only one round of selection using this screening, suggesting this screening method might be useful for selecting aptamers for proteome analysis.

Original languageEnglish
Pages (from-to)1377-1381
Number of pages5
JournalBiotechnology Letters
Volume28
Issue number17
DOIs
Publication statusPublished - 2006 Sep 1

Keywords

  • Aptamer
  • Electrophoresis blotting
  • Multiple target proteins
  • Systematic evolution of ligands by exponential enrichment

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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