We established two monoclonal antibodies (MAbs, A6-1-E4, IgM; א and BC-D1, IgG1, λ), that specifically recognize different epitopes of rat Δ3, Δ2-enoyl-CoA isomerase (ECI). We then developed a specific and reliable sandwich enzyme-linked immunosorbent assay (sandwich ELISA) using a capture monoclonal antibody and an indicator polyclonal antibody to evaluate ECI. The amounts of ECI were linearly determined in the ranges from 15 to 250 ng/well by this method. This assay can detect the amount of ECI in tissue homogenates without subfractionation. The ECI levels were 0.13 ng/μg homogenate in normal rat liver and 1.50 ng/μg homogenate in clofibrate-treated rat liver.
- Monoclonal antibody
- Sandwich ELISA
- Δ, Δ-enoyl-CoA-isomerase
- β-oxidation of unsaturated fatty acids
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)