A sandwich enzyme-linked imrmmosorbent assay for Δ3, Δ2-enoyl-CoA isomerase in rat-tissue homogenates

Hideaki Suzuki, Yoshihisa Tomioka, Shunji Ishiwata, Takahumi Yamaguchi, Koichi Miura, Takawori Hishinuma, Michinao Mizugaki

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

We established two monoclonal antibodies (MAbs, A6-1-E4, IgM; א and BC-D1, IgG1, λ), that specifically recognize different epitopes of rat Δ3, Δ2-enoyl-CoA isomerase (ECI). We then developed a specific and reliable sandwich enzyme-linked immunosorbent assay (sandwich ELISA) using a capture monoclonal antibody and an indicator polyclonal antibody to evaluate ECI. The amounts of ECI were linearly determined in the ranges from 15 to 250 ng/well by this method. This assay can detect the amount of ECI in tissue homogenates without subfractionation. The ECI levels were 0.13 ng/μg homogenate in normal rat liver and 1.50 ng/μg homogenate in clofibrate-treated rat liver.

Original languageEnglish
Pages (from-to)35-45
Number of pages11
JournalTohoku Journal of Experimental Medicine
Volume179
Issue number1
DOIs
Publication statusPublished - 1996 May

Keywords

  • Monoclonal antibody
  • Sandwich ELISA
  • Δ, Δ-enoyl-CoA-isomerase
  • β-oxidation of unsaturated fatty acids

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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