A role for RAD54B in homologous recombination in human cells

Kiyoshi Miyagawa, Takanori Tsuruga, Aiko Kinomura, Kiyomi Usui, Mari Katsura, Satoshi Tashiro, Hiromu Mishima, Kozo Tanaka

Research output: Contribution to journalArticlepeer-review

60 Citations (Scopus)

Abstract

In human somatic cells, homologous recombination is a rare event. To facilitate the targeted modification of the genome for research and gene therapy applications, efforts should be directed toward understanding the molecular mechanisms of homologous recombination in human cells. Although human genes homologous to members of the RAD52 epistasis group in yeast have been identified, no genes have been demonstrated to play a role in homologous recombination in human cells. Here, we report that RAD54B plays a critical role in targeted integration in human cells. Inactivation of RAD54B in a colon cancer cell line resulted in severe reduction of targeted integration frequency. Sensitivity to DNA-damaging agents and sister-chromatid exchange were not affected in RAD54B-deficient cells. Parts of these phenotypes were similar to those of Saccharomyces cerevisiae tid1/rdh54 mutants, suggesting that RAD54B may be a human homolog of TID1/RDH54. In yeast, TID1/ RDH54 acts in the recombinational repair pathway via roles partially overlapping those of RAD54. Our findings provide the first genetic evidence that the mitotic recombination pathway is functionally conserved from yeast to humans.

Original languageEnglish
Pages (from-to)175-180
Number of pages6
JournalEMBO Journal
Volume21
Issue number1-2
DOIs
Publication statusPublished - 2002 Jan 15

Keywords

  • Homologous recombination
  • Human cells
  • Mitosis
  • Sister-chromatid exchange
  • Targeted integration

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Fingerprint Dive into the research topics of 'A role for RAD54B in homologous recombination in human cells'. Together they form a unique fingerprint.

Cite this