A retinoic acid receptor-selective agonist causes jaw deformity in the Japanese flounder, Paralichthys olivaceus

Y. Haga, T. Suzuki, H. Kagechika, T. Takeuchi

Research output: Contribution to journalArticlepeer-review

61 Citations (Scopus)

Abstract

We examined the effects of synthetic retinoids on the jaw development of Japanese flounder during postembryonic development. Flounder larvae were exposed to Am80 [a retinoic acid receptor (RAR)α/β-selective retinoid] and methoprene acid [MA, a retinoid X receptor (RXR)-selective retinoid]. Am80 induced lower jaw deformities in all fish, whereas MA induced lower jaw deformities in less than 20% of the fish. Am80 caused drastic deformities in the upper jaw as well as the lower jaw, while MA did not affect the upper jaw. RT-PCR analysis of the lower jaw revealed that RAR expression was greatly increased by Am80 treatment. Expression of patched (ptc) which is a receptor of the hedgehog family, was inhibited by both Am80 and MA treatments. Immunohistochemistry using anti-RARα-antibody revealed that RARα-positive osteoblasts exist around the jaw bones. We propose that disturbance of the RAR signaling pathway regulating osteoblast activity is mainly responsible for the jaw deformities.

Original languageEnglish
Pages (from-to)381-392
Number of pages12
JournalAquaculture
Volume221
Issue number1-4
DOIs
Publication statusPublished - 2003 May 1
Externally publishedYes

Keywords

  • Deformity
  • Japanese flounder
  • Jaw
  • Osteoblast
  • RAR
  • Retinoid

ASJC Scopus subject areas

  • Aquatic Science

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