A Receptor Guanylate Cyclase, Gyc76C, Mediates Humoral, and Cellular Responses in Distinct Ways in Drosophila Immunity

Shinzo Iwashita, Hiroaki Suzuki, Akira Goto, Tomohito Oyama, Hirotaka Kanoh, Takayuki Kuraishi, Naoyuki Fuse, Tamaki Yano, Yoshiteru Oshima, Julian A.T. Dow, Shireen Anne Davies, Shoichiro Kurata

Research output: Contribution to journalArticlepeer-review

Abstract

Innate immunity is an evolutionarily conserved host defense system against infections. The fruit fly Drosophila relies solely on innate immunity for infection defense, and the conservation of innate immunity makes Drosophila an ideal model for understanding the principles of innate immunity, which comprises both humoral and cellular responses. The mechanisms underlying the coordination of humoral and cellular responses, however, has remained unclear. Previously, we identified Gyc76C, a receptor-type guanylate cyclase that produces cyclic guanosine monophosphate (cGMP), as an immune receptor in Drosophila. Gyc76C mediates the induction of antimicrobial peptides for humoral responses by a novel cGMP pathway including a membrane-localized cGMP-dependent protein kinase, DG2, through downstream components of the Toll receptor such as dMyD88. Here we show that Gyc76C is also required for the proliferation of blood cells (hemocytes) for cellular responses to bacterial infections. In contrast to Gyc76C-dependent antimicrobial peptide induction, Gyc76C-dependent hemocyte proliferation is meditated by a small GTPase, Ras85D, and not by DG2 or dMyD88, indicating that Gyc76C mediates the cellular and humoral immune responses in distinct ways.

Original languageEnglish
Article number35
JournalFrontiers in immunology
Volume11
DOIs
Publication statusPublished - 2020 Jan 28
Externally publishedYes

Keywords

  • Drosophila
  • cellular immune responses
  • humoral immune responses
  • innate immunity
  • receptor-type guanylate cyclase

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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