A rapid flow mixer with 11-μs mixing time microfabricated by a pulsed-laser ablation technique: Observation of a barrier-limited collapse in cytochrome c folding

Shuzo Matsumoto, Akira Yane, Satoru Nakashima, Masaki Hashida, Masayuki Fujita, Yuji Goto, Satoshi Takahashi

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Proteins with more than ∼100 residues usually demonstrate a rapid and significant collapse as the earliest event in their folding dynamics; however, the limit in the time resolution of available experimental methods precluded us from conducting a detailed analysis of the event. To investigate the collapse in a real time, a new rapid flow mixer was developed by using a femtosecond laser ablation technique, which achieved a mixing time of 11 μs. By using the developed mixer, the collapse dynamics of cytochrome c, initiated by pH jumps after the solution mixing, was investigated. The time domain within 100 μs, which was previously unobservable, was clearly resolved and demonstrated that the process can be approximated by a single-exponential process. The results support the presence of a single kinetic barrier in the initial collapse of cytochrome c.

Original languageEnglish
Pages (from-to)3840-3841
Number of pages2
JournalJournal of the American Chemical Society
Volume129
Issue number13
DOIs
Publication statusPublished - 2007 Apr 4
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Fingerprint Dive into the research topics of 'A rapid flow mixer with 11-μs mixing time microfabricated by a pulsed-laser ablation technique: Observation of a barrier-limited collapse in cytochrome c folding'. Together they form a unique fingerprint.

  • Cite this