TY - JOUR
T1 - A protein/antibiotic releasing poly(lactic-co-glycolic acid)/lecithin scaffold for bone repair applications
AU - Shi, Xuetao
AU - Wang, Yingjun
AU - Ren, Li
AU - Huang, Wei
AU - Wang, Dong An
N1 - Funding Information:
Xuetao Shi was a China Scholarship Council (CSC) scholarship recipient (2007U33046). This research was financially supported by the National Basic Research Program of China (Grant 2005CB623902), Key Projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period (2006BA116B04), the State Key Program of National Natural Science of China (50732003), and also supported by AcRF Tier 1 RG 64/08, Ministry of Education, Singapore.
Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2009/5/21
Y1 - 2009/5/21
N2 - Novel poly(lactic-co-glycolic acid) (PLGA)-hybridizing-lecithin scaffolds loaded with drug or protein were prepared with water/oil/water techniques and sintering microspheres technique. In such fabricated composite scaffolds (abbreviated "PLGA/Lec-SMS"), the introduction of lecithin component has been proven capable of largely enhancing Gentamicin (GS) and protein (Bovine Serum Albumin) encapsulation efficiency. The in vitro GS and BSA releasing profiles of PLGA/Lec-SMS system were plotted basing over 60 days' and 18 days' data collection, respectively. It indicates a sustained releasing tendency despite a burst at the very beginning. The antibacterial properties of GS-laden scaffolds were determined in vitro, and the antibacterial activity of scaffolds was enhanced by incorporating lecithin into PLGA bulks. Additionally, mesenchymal stem cells (MSCs) were seeded onto PLGA-SMS and PLGA/Lec-SMS in vitro. The outcome confirmed PLGA/Lec(5%)-SMS functions to improve MSC proliferation and also to enhance general ALP production and calcium secretion which is the vital markers for osteogenesis. In conclusion, this newly designed antibiotic releasing PLGA/Lec-SMS is promising for bone-repairing therapeutics.
AB - Novel poly(lactic-co-glycolic acid) (PLGA)-hybridizing-lecithin scaffolds loaded with drug or protein were prepared with water/oil/water techniques and sintering microspheres technique. In such fabricated composite scaffolds (abbreviated "PLGA/Lec-SMS"), the introduction of lecithin component has been proven capable of largely enhancing Gentamicin (GS) and protein (Bovine Serum Albumin) encapsulation efficiency. The in vitro GS and BSA releasing profiles of PLGA/Lec-SMS system were plotted basing over 60 days' and 18 days' data collection, respectively. It indicates a sustained releasing tendency despite a burst at the very beginning. The antibacterial properties of GS-laden scaffolds were determined in vitro, and the antibacterial activity of scaffolds was enhanced by incorporating lecithin into PLGA bulks. Additionally, mesenchymal stem cells (MSCs) were seeded onto PLGA-SMS and PLGA/Lec-SMS in vitro. The outcome confirmed PLGA/Lec(5%)-SMS functions to improve MSC proliferation and also to enhance general ALP production and calcium secretion which is the vital markers for osteogenesis. In conclusion, this newly designed antibiotic releasing PLGA/Lec-SMS is promising for bone-repairing therapeutics.
KW - Antibacterial
KW - Controlled release
KW - Lecithin
KW - Poly(lactide-co-glycolide)
KW - Scaffold
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U2 - 10.1016/j.ijpharm.2009.02.013
DO - 10.1016/j.ijpharm.2009.02.013
M3 - Article
C2 - 19429292
AN - SCOPUS:67349170473
VL - 373
SP - 85
EP - 92
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
SN - 0378-5173
IS - 1-2
ER -