TY - JOUR
T1 - A pressure-driven column-based technique for the efficient extraction of DNA from respiratory samples
AU - Hirama, Takashi
AU - Mogi, Hajime
AU - Egashira, Hitoshi
AU - Yamamoto, Eiji
AU - Kukisaki, Shigenari
AU - Hagiwara, Koichi
AU - Takei, Osamu
N1 - Funding Information:
The authors have declared that no competing interests exist. This work was supported by the Adaptable and Seamless Technology Transfer Program through Target-driven R&D ( AS2321117F ) from the Japan Science and Technology Agency and Grant-in-Aid for Young Scientists (B) (No. 23790918 ) from the Japan Society for the Promotion of Science. No additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. In addition, the manuscript has been reviewed by all of the authors and is not being submitted to any other journals. All of the authors have declared that no competing interests exist.
Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2015/5/1
Y1 - 2015/5/1
N2 - Currently molecular techniques are a broadly accepted tool for diagnosis and are able to benefit patients in clinical practice. The polymerase chain reaction (PCR) has been especially incorporated into practical applications that are already in widespread use across the globe. With regard to the initial DNA extraction from clinically relevant samples, a number of commercially available kits are commonly used and are also designed to be easy to handle and less labor-intensive. In this study, the pressure system extracting DNA in column-based kit was developed, and its utility was compared with the centrifuge method using sputum from patients who were diagnosed with pneumonia. Also, due to the compact size and rapid processing time, the practical application of the pressure-based system incorporated into an automated pipetting machine was evaluated through clinical study. Our data suggests that DNA extraction by pressure was capable of serving as a substitute for the centrifuge method, and the compact and automatic nature of the pressure system device provided rapid and valuable information for clinical practice.
AB - Currently molecular techniques are a broadly accepted tool for diagnosis and are able to benefit patients in clinical practice. The polymerase chain reaction (PCR) has been especially incorporated into practical applications that are already in widespread use across the globe. With regard to the initial DNA extraction from clinically relevant samples, a number of commercially available kits are commonly used and are also designed to be easy to handle and less labor-intensive. In this study, the pressure system extracting DNA in column-based kit was developed, and its utility was compared with the centrifuge method using sputum from patients who were diagnosed with pneumonia. Also, due to the compact size and rapid processing time, the practical application of the pressure-based system incorporated into an automated pipetting machine was evaluated through clinical study. Our data suggests that DNA extraction by pressure was capable of serving as a substitute for the centrifuge method, and the compact and automatic nature of the pressure system device provided rapid and valuable information for clinical practice.
KW - Column
KW - DNA extraction
KW - HIRA-TAN
KW - Pressure
KW - Real-time PCR
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U2 - 10.1016/j.cca.2015.03.023
DO - 10.1016/j.cca.2015.03.023
M3 - Article
C2 - 25824633
AN - SCOPUS:84964310691
SN - 0009-8981
VL - 445
SP - 122
EP - 126
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
ER -