A pressure-driven column-based technique for the efficient extraction of DNA from respiratory samples

Takashi Hirama, Hajime Mogi, Hitoshi Egashira, Eiji Yamamoto, Shigenari Kukisaki, Koichi Hagiwara, Osamu Takei

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Currently molecular techniques are a broadly accepted tool for diagnosis and are able to benefit patients in clinical practice. The polymerase chain reaction (PCR) has been especially incorporated into practical applications that are already in widespread use across the globe. With regard to the initial DNA extraction from clinically relevant samples, a number of commercially available kits are commonly used and are also designed to be easy to handle and less labor-intensive. In this study, the pressure system extracting DNA in column-based kit was developed, and its utility was compared with the centrifuge method using sputum from patients who were diagnosed with pneumonia. Also, due to the compact size and rapid processing time, the practical application of the pressure-based system incorporated into an automated pipetting machine was evaluated through clinical study. Our data suggests that DNA extraction by pressure was capable of serving as a substitute for the centrifuge method, and the compact and automatic nature of the pressure system device provided rapid and valuable information for clinical practice.

Original languageEnglish
Pages (from-to)122-126
Number of pages5
JournalClinica Chimica Acta
Volume445
DOIs
Publication statusPublished - 2015 May 1
Externally publishedYes

Keywords

  • Column
  • DNA extraction
  • HIRA-TAN
  • Pressure
  • Real-time PCR

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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