A preadipocyte clonal line from bovine intramuscular adipose tissue: Nonexpression of glut-4 protein during adipocyte differentiation

Hisashi Aso, Hiroyuki Abe, Ikuyo Nakajima, Kyouhei Ozutsumi, Takahiro Yamaguchi, Yasushi Takamori, Akira Kodama, Fuminori B. Hoshino, Shoichi Takano

Research output: Contribution to journalArticlepeer-review

69 Citations (Scopus)

Abstract

A clonal bovine intramuscular preadipocyte (BIP) line has been established from the intramuscular white adipose tissue of the M. longissimus thoracis in each of three Japanese Black cattle. Exponentially growing BIP cells exhibited a fibroblastic appearance. Adipocyte differentiation was initiated by treating confluent BIP cells with differentiation medium containing insulin and dexamethasone. Small lipid droplets appeared 5-6 days after stimulation and occupied a large fraction of the cell volume at 10 days and beyond. During the adipose conversion, the incorporation of acetate to the cells gradually increased by 10-fold and reached a maximum at day 5. However, incorporation of glucose increased only S-folds prior to this conversion, even though GLUT-1 level increased by 13-fold at day 7. GLUT-4 on the other hand, was not detected during the course of differentiation. These results suggested that adipose tissue metabolisms in ruminants were different from that of non-ruminants.

Original languageEnglish
Pages (from-to)369-375
Number of pages7
JournalBiochemical and biophysical research communications
Volume213
Issue number2
DOIs
Publication statusPublished - 1995 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'A preadipocyte clonal line from bovine intramuscular adipose tissue: Nonexpression of glut-4 protein during adipocyte differentiation'. Together they form a unique fingerprint.

Cite this