A possible translational control of 3-hydroxy-3-methylglutaryl coenzyme A reductase induction by ML-236B(Compactin) in isolated rat hepatocytes

Junji Koizumi, Hiroshi Mabuchi, Ryoyu Takeda, Hiroshi Okamoto

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

ML-236B ("Compactin"), a competitive inhibitor of 3-hydroxy-3-methylglutaryl(HMG)-CoA reductase, increased the cholesterol synthesis and the HMG-CoA reductase activity in isolated rat hepatocytes. These increases were prevented by 0.2 mM puromycin, but not by 10 μg/ml actinomycin D and 40 μg/ml α-amanitin. These results indicated that the increases in cholesterol synthesis and HMG-CoA reductase activity by ML-236B required the enzyme synthesis but not newly synthesized mRNA. The regulatory site of feed-back inhibition by cholesterol for the HMG-CoA reductase synthesis in liver may be at the translational level.

Original languageEnglish
Pages (from-to)240-246
Number of pages7
JournalBiochemical and biophysical research communications
Volume108
Issue number1
DOIs
Publication statusPublished - 1982 Sep 16
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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