TY - JOUR
T1 - A possible role of Atg8 homologs as a scaffold for signal transduction
AU - Itoh, Takashi
AU - Fukuda, Mitsunori
N1 - Funding Information:
served membrane trafficsmall GTPases are evol%king putionarProteinsily conO, en-juPzymatgation sUic intysteEeractiom. TJns bheTetwse phUeen RysicSal aJab3ndC3B, /or VTechEducUnoloatioFgy (MEXT) of Japan (to T.I.n, Culture, Sports and and their activity is regulated by two key the Atg12-5-16L1 complex, Atg8 homo-and M.F.) and the Uehara Memorial enzymes, a guanine nucleotide exchange logs and OATL1 lead us to hypothesize Foundation (to T.I.) for funding this work.
PY - 2011/9
Y1 - 2011/9
N2 - Atg8 and its homologs are essential for autophagosome formation in various species. In animal cells, Atg8 homologs have an additional function in clearance of damaged organelles and bacteria, acting as a landmark for selective autophagy. We have recently shown that OATL1, a Rab-GTPase-activating protein (Rab-GAP), is a novel binding partner of Atg8 homologs in mammalian cells, but to our surprise, it is not a substrate of autophagy. Further analysis indicates that OATL1 is involved in the fusion between autophagosomes and lysosomes through its GAP activity and its Atg8 homolog binding activity. Our findings suggest a novel function of Atg8 homologs as a scaffold for signal transduction that regulates autophagosomal maturation.
AB - Atg8 and its homologs are essential for autophagosome formation in various species. In animal cells, Atg8 homologs have an additional function in clearance of damaged organelles and bacteria, acting as a landmark for selective autophagy. We have recently shown that OATL1, a Rab-GTPase-activating protein (Rab-GAP), is a novel binding partner of Atg8 homologs in mammalian cells, but to our surprise, it is not a substrate of autophagy. Further analysis indicates that OATL1 is involved in the fusion between autophagosomes and lysosomes through its GAP activity and its Atg8 homolog binding activity. Our findings suggest a novel function of Atg8 homologs as a scaffold for signal transduction that regulates autophagosomal maturation.
KW - Atg8 homologs
KW - Autophagosomal maturation
KW - GTPase-activating protein
KW - OATL1
KW - Rab33B
UR - http://www.scopus.com/inward/record.url?scp=80052374192&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80052374192&partnerID=8YFLogxK
U2 - 10.4161/auto.7.9.16178
DO - 10.4161/auto.7.9.16178
M3 - Article
C2 - 21597320
AN - SCOPUS:80052374192
VL - 7
SP - 1080
EP - 1081
JO - Autophagy
JF - Autophagy
SN - 1554-8627
IS - 9
ER -