A pollen coat-inducible autoinhibited Ca2+-ATPase expressed in stigmatic papilla cells is required for compatible pollination in the brassicaceae

Megumi Iwano, Motoko Igarashi, Yoshiaki Tarutani, Pulla Kaothien-Nakayama, Hideki Nakayama, Hideki Moriyama, Ryo Yakabe, Tetsuyuki Entani, Hiroko Shimosato-Asano, Masao Ueki, Gen Tamiya, Seiji Takayama

    Research output: Contribution to journalArticlepeer-review

    37 Citations (Scopus)

    Abstract

    In the Brassicaceae, intraspecific non-self pollen (compatible pollen) can germinate and grow into stigmatic papilla cells, while self-pollen or interspecific pollen is rejected at this stage. However, the mechanisms underlying this selective acceptance of compatible pollen remain unclear. Here, using a cell-impermeant calcium indicator, we showed that the compatible pollen coat contains signaling molecules that stimulate Ca2+ export from the papilla cells. Transcriptome analyses of stigmas suggested that autoinhibited Ca2+-ATPase13 (ACA13) was induced after both compatible pollination and compatible pollen coat treatment. A complementation test using a yeast Saccharomyces cerevisiae strain lacking major Ca2+ transport systems suggested that ACA13 indeed functions as an autoinhibited Ca2+ transporter. ACA13 transcription increased in papilla cells and in transmitting tracts after pollination. ACA13 protein localized to the plasma membrane and to vesicles near the Golgi body and accumulated at the pollen tube penetration site after pollination. The stigma of a T-DNA insertion line of ACA13 exhibited reduced Ca2+ export, as well as defects in compatible pollen germination and seed production. These findings suggest that stigmatic ACA13 functions in the export of Ca2+ to the compatible pollen tube, which promotes successful fertilization.

    Original languageEnglish
    Pages (from-to)636-649
    Number of pages14
    JournalPlant Cell
    Volume26
    Issue number2
    DOIs
    Publication statusPublished - 2014 Feb

    ASJC Scopus subject areas

    • Plant Science
    • Cell Biology

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