A point mutation of C-KI-RAS gene was found in human esophageal carcinoma cell lines but not in primary esophageal carcinomas

Chizuko Shiga, Kiyoto Shiga, Hironobu Sasano, Shozo Mori

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Activation of the ras oncogene in primary human esophageal carcinomas and cell lines established from such carcinomas was analyzed using the polymerase chain reaction (PCR)-direct sequencing method. This analysis revealed a GC→AT transition at the second base in the 12th codon of the c-Ki-ras gene in TE1 and TE2 esophageal carcinoma cell lines. In contrast, no point mutation was detected in the 12th, 13th, and 61st codon of the c-Ki-ras and c-Ha-ras gene in 31 primary esophageal carcinomas including those from which TE1 and TE2 cell lines were established. These results demonstrate that while activation of the c-Ki-ras gene by point mutation occurred in a subset of esophageal carcinoma cell lines during establishment of the cell lines, the activation events are not important in the transformation of human esophageal epithelial cells.

Original languageEnglish
Pages (from-to)515-521
Number of pages7
JournalBiochemical and biophysical research communications
Volume187
Issue number1
DOIs
Publication statusPublished - 1992 Aug 31

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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