TY - JOUR
T1 - A plant u-box protein, pub4, regulates asymmetric cell division and cell proliferation in the root meristem
AU - Kinoshita, Atsuko
AU - Ten Hove, Colette A.
AU - Tabata, Ryo
AU - Yamada, Masashi
AU - Shimizu, Noriko
AU - Ishida, Takashi
AU - Yamaguchi, Katsushi
AU - Shigenobu, Shuji
AU - Takebayashi, Yumiko
AU - Iuchi, Satoshi
AU - Kobayashi, Masatomo
AU - Kurata, Tetsuya
AU - Wada, Takuji
AU - Seo, Mitsunori
AU - Hasebe, Mitsuyasu
AU - Blilou, Ikram
AU - Fukuda, Hiroo
AU - Scheres, Ben
AU - Heidstra, Renze
AU - Kamiya, Yuji
AU - Sawa, Shinichiro
PY - 2015/2/1
Y1 - 2015/2/1
N2 - The root meristem (RM) is a fundamental structure that is responsible for postembryonic root growth. The RMc ontains the quiescent center (QC), stem cells and frequently dividing meristematic cells, in which the timing and the frequency of cell division are tightly regulated. In Arabidopsis thaliana, several gain-of-function analyses have demonstrated that peptide ligands of the CLAVATA3 (CLV3)/EMBRYO SURROUNDING REGION-RELATED (CLE) family are important for maintaining RM size. Here, we demonstrate that a plant U-box E3 ubiquitin ligase, PUB4, isanovel downstream component of CLV3/CLE signaling in the RM. Mutations in PUB4 reduced the inhibitory effect of exogenous CLV3/CLE peptide on root cell proliferation and columella stem cell maintenance. Moreover, pub4 mutants grown without exogenous CLV3/CLE peptide exhibited characteristic phenotypes in the RM, such as enhanced root growth, increased number of cortex/endodermis stem cells and decreased number of columella layers. Our phenotypic and gene expression analyses indicated that PUB4 promotes expression of a cell cycle regulatory gene, CYCD6;1, and regulates formative periclinal asymmetric cell divisions in endodermis and cortex/ endodermis initial daughters. These data suggest that PUB4 functions as a global regulator of cell proliferation and the timing of asymmetric cell division that are important for final root architecture.
AB - The root meristem (RM) is a fundamental structure that is responsible for postembryonic root growth. The RMc ontains the quiescent center (QC), stem cells and frequently dividing meristematic cells, in which the timing and the frequency of cell division are tightly regulated. In Arabidopsis thaliana, several gain-of-function analyses have demonstrated that peptide ligands of the CLAVATA3 (CLV3)/EMBRYO SURROUNDING REGION-RELATED (CLE) family are important for maintaining RM size. Here, we demonstrate that a plant U-box E3 ubiquitin ligase, PUB4, isanovel downstream component of CLV3/CLE signaling in the RM. Mutations in PUB4 reduced the inhibitory effect of exogenous CLV3/CLE peptide on root cell proliferation and columella stem cell maintenance. Moreover, pub4 mutants grown without exogenous CLV3/CLE peptide exhibited characteristic phenotypes in the RM, such as enhanced root growth, increased number of cortex/endodermis stem cells and decreased number of columella layers. Our phenotypic and gene expression analyses indicated that PUB4 promotes expression of a cell cycle regulatory gene, CYCD6;1, and regulates formative periclinal asymmetric cell divisions in endodermis and cortex/ endodermis initial daughters. These data suggest that PUB4 functions as a global regulator of cell proliferation and the timing of asymmetric cell division that are important for final root architecture.
KW - Arabidopsis development
KW - Asymmetric cell division
KW - Cell proliferation
KW - Root meristem
UR - http://www.scopus.com/inward/record.url?scp=84921475875&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84921475875&partnerID=8YFLogxK
U2 - 10.1242/dev.113167
DO - 10.1242/dev.113167
M3 - Article
C2 - 25605779
AN - SCOPUS:84921475875
VL - 142
SP - 444
EP - 453
JO - Development (Cambridge)
JF - Development (Cambridge)
SN - 0950-1991
IS - 3
ER -