TY - JOUR
T1 - A Phox2b bac transgenic rat line useful for understanding respiratory rhythm generator neural circuitry
AU - Ikeda, Keiko
AU - Takahashi, Masanori
AU - Sato, Shigeru
AU - Igarashi, Hiroyuki
AU - Ishizuka, Toru
AU - Yawo, Hiromu
AU - Arata, Satoru
AU - Southard-Smith, E. Michelle
AU - Kawakami, Kiyoshi
AU - Onimaru, Hiroshi
N1 - Funding Information:
We thank all members of Division of Biology of Jichi Medical University for technical assistance. The establishment of LE-Tg(Gt(ROSA)26Sor/CAG-tdTomato)24Jfhy was supported by a Grant-in-Aid for Scientific Research on Innovative Areas (Comprehensive Brain Science Network) from the Ministry of Education, Science, Sports and Culture of Japan. The authors declare no conflict of interest.
Publisher Copyright:
© 2015 Ikeda et al.
PY - 2015/7/6
Y1 - 2015/7/6
N2 - The key role of the respiratory neural center is respiratory rhythm generation to maintain homeostasis through the control of arterial blood pCO2/pH and pO2levels. The neuronal network responsible for respiratory rhythm generation in neonatal rat resides in the ventral side of the medulla and is composed of two groups; the parafacial respiratory group (pFRG) and the pre-Bötzinger complex group (preBötC). The pFRG partially overlaps in the retrotra-pezoid nucleus (RTN), which was originally identified in adult cats and rats. Part of the pre-inspiratory (Pre-I) neurons in the RTN/pFRG serves as central chemoreceptor neurons and the CO2sensitive Pre-I neurons express homeobox gene Phox2b. Phox2b encodes a transcription factor and is essential for the development of the sensory-motor visceral circuits. Mutations in human PHOX2B cause congenital hypoventilation syndrome, which is characterized by blunted ventilatory response to hypercapnia. Here we describe the generation of a novel transgenic (Tg) rat harboring fluorescently labeled Pre-I neurons in the RTN/pFRG. In addition, the Tg rat showed fluorescent signals in autonomic enteric neurons and carotid bodies. Because the Tg rat expresses inducible Cre recombinase in PHOX2B-positive cells during development, it is a potentially powerful tool for dissecting the entire picture of the respiratory neural network during development and for identifying the CO2/O2sensor molecules in the adult central and peripheral nervous systems.
AB - The key role of the respiratory neural center is respiratory rhythm generation to maintain homeostasis through the control of arterial blood pCO2/pH and pO2levels. The neuronal network responsible for respiratory rhythm generation in neonatal rat resides in the ventral side of the medulla and is composed of two groups; the parafacial respiratory group (pFRG) and the pre-Bötzinger complex group (preBötC). The pFRG partially overlaps in the retrotra-pezoid nucleus (RTN), which was originally identified in adult cats and rats. Part of the pre-inspiratory (Pre-I) neurons in the RTN/pFRG serves as central chemoreceptor neurons and the CO2sensitive Pre-I neurons express homeobox gene Phox2b. Phox2b encodes a transcription factor and is essential for the development of the sensory-motor visceral circuits. Mutations in human PHOX2B cause congenital hypoventilation syndrome, which is characterized by blunted ventilatory response to hypercapnia. Here we describe the generation of a novel transgenic (Tg) rat harboring fluorescently labeled Pre-I neurons in the RTN/pFRG. In addition, the Tg rat showed fluorescent signals in autonomic enteric neurons and carotid bodies. Because the Tg rat expresses inducible Cre recombinase in PHOX2B-positive cells during development, it is a potentially powerful tool for dissecting the entire picture of the respiratory neural network during development and for identifying the CO2/O2sensor molecules in the adult central and peripheral nervous systems.
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U2 - 10.1371/journal.pone.0132475
DO - 10.1371/journal.pone.0132475
M3 - Article
C2 - 26147470
AN - SCOPUS:84938363998
VL - 10
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 7
M1 - e0132475
ER -