Abstract
We propose a new method of synthesizing perfect sets of probe deoxyribonucleic acid (DNA) on a chip to produce high-performance DNA chips. Two different methods of direct synthesis are combined to generate single strands of probe DNA on the chip's surface. The first is an area-specific photosensitive synthesis of DNA that exploits photolithographic techniques. The other is a non-photosensitive synthesis of DNA. This is a much more reliable method of synthesis and is used for the remaining sequences of probe DNA, i.e. those outside the photosynthesis region. In the synthesis procedure, photosensitive reagents are only used to make certain variations to the probe DNA and areas with several kinds of probe DNA (4 to 16 nucleotides in length) were synthesized on each 50 μm2 of the probe. We examined the application of our DNA chip to single-nucleotide polymorphism (SNP) analysis, which is used to check for the mutant promoters of liver cancer. We found that no non-specific signals from a hybridization reaction of the sample DNA variants appeared and that this DNA chip successfully discriminated slight differences in genomic (DNA) information at the single base-pair level.
Original language | English |
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Pages (from-to) | 67-76 |
Number of pages | 10 |
Journal | Sensors and Actuators, B: Chemical |
Volume | 83 |
Issue number | 1-3 |
DOIs | |
Publication status | Published - 2002 Mar 15 |
Externally published | Yes |
Event | Selected papers from Transduckers '01 Eurosensors XV (Transduckers 2001) - Munich, Germany Duration: 2001 Jun 10 → 2001 Jun 14 |
Keywords
- DNA chip
- Photochemical synthesis
- Photolithography
- Photosensitive reagent
- Polymorphism
- SNP analysis
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Instrumentation
- Condensed Matter Physics
- Surfaces, Coatings and Films
- Metals and Alloys
- Electrical and Electronic Engineering
- Materials Chemistry