A photochemical/chemical direct method of synthesizing high-performance deoxyribonucleic acid chips for rapid and parallel gene analysis

Kazunori Takahashii, Kohji Seio, Mitsuo Sekine, Okio Hino, Masayoshi Esashi

Research output: Contribution to journalConference articlepeer-review

6 Citations (Scopus)

Abstract

We propose a new method of synthesizing perfect sets of probe deoxyribonucleic acid (DNA) on a chip to produce high-performance DNA chips. Two different methods of direct synthesis are combined to generate single strands of probe DNA on the chip's surface. The first is an area-specific photosensitive synthesis of DNA that exploits photolithographic techniques. The other is a non-photosensitive synthesis of DNA. This is a much more reliable method of synthesis and is used for the remaining sequences of probe DNA, i.e. those outside the photosynthesis region. In the synthesis procedure, photosensitive reagents are only used to make certain variations to the probe DNA and areas with several kinds of probe DNA (4 to 16 nucleotides in length) were synthesized on each 50 μm2 of the probe. We examined the application of our DNA chip to single-nucleotide polymorphism (SNP) analysis, which is used to check for the mutant promoters of liver cancer. We found that no non-specific signals from a hybridization reaction of the sample DNA variants appeared and that this DNA chip successfully discriminated slight differences in genomic (DNA) information at the single base-pair level.

Original languageEnglish
Pages (from-to)67-76
Number of pages10
JournalSensors and Actuators, B: Chemical
Volume83
Issue number1-3
DOIs
Publication statusPublished - 2002 Mar 15
EventSelected papers from Transduckers '01 Eurosensors XV (Transduckers 2001) - Munich, Germany
Duration: 2001 Jun 102001 Jun 14

Keywords

  • DNA chip
  • Photochemical synthesis
  • Photolithography
  • Photosensitive reagent
  • Polymorphism
  • SNP analysis

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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