A nucleoporin is required for induction of Ca2+ spiking in legume nodule development and essential for rhizobial and fungal symbiosis

Norihito Kanamori, Lene Heegaard Madsen, Simona Radutoiu, Mirela Frantescu, Esben M.H. Quistgaard, Hiroki Miwa, J. Allan Downie, Euan K. James, Hubert H. Felle, Line Lindegaard Haaning, Torben Heick Jensen, Shusei Sato, Yasukazu Nakamura, Satoshi Tabata, Niels Sandal, Jens Stougaard

Research output: Contribution to journalArticlepeer-review

277 Citations (Scopus)


Nuclear-cytoplasmic partitioning and traffic between cytoplasmic and nuclear compartments are fundamental processes in eukaryotic cells. Nuclear pore complexes mediate transport of proteins, RNAs and ribonucleoprotein particles in and out of the nucleus. Here we present positional cloning of a plant nucleoporin gene. Nup133, essential for a symbiotic signal transduction pathway shared by Rhizobium bacteria and mycorrhizal fungi. Mutation of Nup133 results in a temperature sensitive nodulation deficient phenotype and absence of mycorrhizal colonization. Root nodules developing with reduced frequency at permissive temperatures are ineffective and electron microscopy show that Rhizobium bacteria are not released from infection threads. Measurement of ion fluxes using a calcium-sensitive dye show that Nup133 is required for the Ca2+ spiking normally detectable within minutes after application of purified rhizobial Nod-factor signal molecules to root hairs. Localization of NUP133 in the nuclear envelope of root cells and root hair cells shown with enhanced yellow fluorescent protein fusion proteins suggests a novel role for NUP133 nucleoporins in a rapid nuclear-cytoplasmic communication after host-plant recognition of symbiotic microbes. Our results identify a component of an intriguing signal process requiring interaction at the cell plasma membrane and at intracellular nuclear and plastid organelle-membranes to induce a second messenger.

Original languageEnglish
Pages (from-to)359-364
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number2
Publication statusPublished - 2006 Jan 10
Externally publishedYes


  • Legume symbiosis
  • Nuclear pore
  • Nucleoporin
  • Plant-microbe interaction

ASJC Scopus subject areas

  • General


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