A novel type of human α-amylase produced in lung carcinoid tumor

Naohiro Tomita; Horii Akira; Doi Sadayuki; Yokouchi Hideoki; Shiosaki Kouichi; Higashiyama Masahiko; Matsuura Nariaki; Ogawa Michio; Mori Takesada; Matsubara Kenichi

doi:10.1016/0378-1119(89)90003-6

A novel type of human α-amylase produced in lung carcinoid tumor

Naohiro Tomita, Horii Akira, Doi Sadayuki, Yokouchi Hideoki, Shiosaki Kouichi, Higashiyama Masahiko, Matsuura Nariaki, Ogawa Michio, Mori Takesada, Matsubara Kenichi

MED - Medical Sciences

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

A novel type of α-amylase was detected in a lung carcinoid tissue after surveying the cDNA library constructed from this tumor mRNA. Nucleotide sequence analysis showed that the amylase expressed in this carcinoid tumor has 13 and 6 amino acid substitutions when compared with salivary amylase (Amy1) and pancreatic amylase (Amy2), respectively. The nucleotide sequence homologies of cDNAs between this carcinoid amylase and amy1, amy2 are 97.5% and 98.2%, respectively. The nucleotide sequence comparison strongly suggests that this new amylase is the product of the amy3 gene that has been detected in human genome [Emi et al., Gene 62 (1988) 229-235].

Original language	English
Pages (from-to)	11-18
Number of pages	8
Journal	Gene
Volume	76
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(89)90003-6
Publication status	Published - 1989 Mar 15

Keywords

Kultschitzky cell
Recombinant DNA
amylase isozyme
cDNA
phage λ vectors

ASJC Scopus subject areas

Genetics

Access to Document

10.1016/0378-1119(89)90003-6

Cite this

@article{ef71d10c071044a6874d6982afd1a1ab,

title = "A novel type of human α-amylase produced in lung carcinoid tumor",

abstract = "A novel type of α-amylase was detected in a lung carcinoid tissue after surveying the cDNA library constructed from this tumor mRNA. Nucleotide sequence analysis showed that the amylase expressed in this carcinoid tumor has 13 and 6 amino acid substitutions when compared with salivary amylase (Amy1) and pancreatic amylase (Amy2), respectively. The nucleotide sequence homologies of cDNAs between this carcinoid amylase and amy1, amy2 are 97.5% and 98.2%, respectively. The nucleotide sequence comparison strongly suggests that this new amylase is the product of the amy3 gene that has been detected in human genome [Emi et al., Gene 62 (1988) 229-235].",

keywords = "Kultschitzky cell, Recombinant DNA, amylase isozyme, cDNA, phage λ vectors",

author = "Naohiro Tomita and Horii Akira and Doi Sadayuki and Yokouchi Hideoki and Shiosaki Kouichi and Higashiyama Masahiko and Matsuura Nariaki and Ogawa Michio and Mori Takesada and Matsubara Kenichi",

note = "Funding Information: We thankD r. M. Tatsuta,K . Kodamaa nd0 . Doi at the Center for Adult Diseases,O saka for sup-plyingt he lung carcinoidt issue.W e also thank Dr. Y. Takahashfio r his advicea ndd iscussionas ndM s. K. Mimura for her excellents ecretariaal ssistance. This work was supportedb y a ScientificR esearch grant to K.M. from the Ministry of Education, Sciencea nd Culture,J apan.",

year = "1989",

month = mar,

day = "15",

doi = "10.1016/0378-1119(89)90003-6",

language = "English",

volume = "76",

pages = "11--18",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - A novel type of human α-amylase produced in lung carcinoid tumor

AU - Tomita, Naohiro

AU - Akira, Horii

AU - Sadayuki, Doi

AU - Hideoki, Yokouchi

AU - Kouichi, Shiosaki

AU - Masahiko, Higashiyama

AU - Nariaki, Matsuura

AU - Michio, Ogawa

AU - Takesada, Mori

AU - Kenichi, Matsubara

N1 - Funding Information: We thankD r. M. Tatsuta,K . Kodamaa nd0 . Doi at the Center for Adult Diseases,O saka for sup-plyingt he lung carcinoidt issue.W e also thank Dr. Y. Takahashfio r his advicea ndd iscussionas ndM s. K. Mimura for her excellents ecretariaal ssistance. This work was supportedb y a ScientificR esearch grant to K.M. from the Ministry of Education, Sciencea nd Culture,J apan.

PY - 1989/3/15

Y1 - 1989/3/15

N2 - A novel type of α-amylase was detected in a lung carcinoid tissue after surveying the cDNA library constructed from this tumor mRNA. Nucleotide sequence analysis showed that the amylase expressed in this carcinoid tumor has 13 and 6 amino acid substitutions when compared with salivary amylase (Amy1) and pancreatic amylase (Amy2), respectively. The nucleotide sequence homologies of cDNAs between this carcinoid amylase and amy1, amy2 are 97.5% and 98.2%, respectively. The nucleotide sequence comparison strongly suggests that this new amylase is the product of the amy3 gene that has been detected in human genome [Emi et al., Gene 62 (1988) 229-235].

AB - A novel type of α-amylase was detected in a lung carcinoid tissue after surveying the cDNA library constructed from this tumor mRNA. Nucleotide sequence analysis showed that the amylase expressed in this carcinoid tumor has 13 and 6 amino acid substitutions when compared with salivary amylase (Amy1) and pancreatic amylase (Amy2), respectively. The nucleotide sequence homologies of cDNAs between this carcinoid amylase and amy1, amy2 are 97.5% and 98.2%, respectively. The nucleotide sequence comparison strongly suggests that this new amylase is the product of the amy3 gene that has been detected in human genome [Emi et al., Gene 62 (1988) 229-235].

KW - Kultschitzky cell

KW - Recombinant DNA

KW - amylase isozyme

KW - cDNA

KW - phage λ vectors

UR - http://www.scopus.com/inward/record.url?scp=0024500739&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024500739&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(89)90003-6

DO - 10.1016/0378-1119(89)90003-6

M3 - Article

C2 - 2701942

AN - SCOPUS:0024500739

VL - 76

SP - 11

EP - 18

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1

ER -

A novel type of human α-amylase produced in lung carcinoid tumor

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this