Abstract
A novel type of α-amylase was detected in a lung carcinoid tissue after surveying the cDNA library constructed from this tumor mRNA. Nucleotide sequence analysis showed that the amylase expressed in this carcinoid tumor has 13 and 6 amino acid substitutions when compared with salivary amylase (Amy1) and pancreatic amylase (Amy2), respectively. The nucleotide sequence homologies of cDNAs between this carcinoid amylase and amy1, amy2 are 97.5% and 98.2%, respectively. The nucleotide sequence comparison strongly suggests that this new amylase is the product of the amy3 gene that has been detected in human genome [Emi et al., Gene 62 (1988) 229-235].
Original language | English |
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Pages (from-to) | 11-18 |
Number of pages | 8 |
Journal | Gene |
Volume | 76 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1989 Mar 15 |
Externally published | Yes |
Keywords
- Kultschitzky cell
- Recombinant DNA
- amylase isozyme
- cDNA
- phage λ vectors
ASJC Scopus subject areas
- Genetics