A novel transgenic mouse model carrying human tribbles related protein 3 (TRB3) gene and its site specific phenotype

Yuto Sakai, Katsumi Fukamachi, Mitsuru Futakuchi, Ichiro Miyoshi, Hiroyuki Tsuda, Masumi Suzui, Hidetoshi Hayashi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Tribbles related protein 3 (TRB3) pseudokinase plays a crucial role in cell proliferation, migration and morphogenesis during development. In our recent study, an introduction of human TRB3 gene into mouse mammary tumor cells caused an increase of proliferation of tumor cells and their nuclear size. In the current study, to examine whether this gene causes de novo morphological changes in a specific organ site we have developed a novel variation of the transgenic mouse model that conditionally expresses human TRB3 (hTRB3) gene using Cre-recombinase (Cre)/loxP recombination system. By injecting hTRB3 transgene construct into pronuclei of mouse embryo, we eventually obtained four hTRB3 mice. The gene expression was controlled by infection of adenovirus-expressing Cre via the tail vein of hTRB3 mouse. In Cre-mediated hTRB3 mouse, expression of the hTRB3 protein was detected in the cytoplasm of hepatocytes in the liver. Expression of this protein was also seen in lymphocytes in the spleen, glomerular endothelial cells, and epithelial cells of collecting duct of the kidney. In hepatocytes of the hTRB3 mouse, nuclear size was significantly greater than that of the wild type mouse, indicating that hTRB3 can play a role at least in part in hepatic morphogenesis. The present animal model may provide a system for evaluation of de novo morphological changes induced by a specific transgene in a specific organ site.

Original languageEnglish
Pages (from-to)1068-1074
Number of pages7
JournalBiological and Pharmaceutical Bulletin
Volume37
Issue number6
DOIs
Publication statusPublished - 2014 Jun
Externally publishedYes

Keywords

  • Cre-recombinase (Cre)/loxP
  • Transgenic mouse
  • Tribbles related protein 3 (TRB3)

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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