A Novel Tool for Detecting Amyloid Deposits in Systemic Amyloidosis in Vitro and in Vivo

Yukio Ando, Katsuki Haraoka, Hisayasu Terazaki, Yutaka Tanoue, Kensuke Ishikawa, Shoichi Katsuragi, Masaaki Nakamura, Xuguo Sun, Kazuko Nakagawa, Kazumi Sasamoto, Kazuhiro Takesako, Takashi Ishizaki, Yutaka Sasaki, Katsumi Doh-Ura

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

We synthesized (trans, trans)-1-bromo-2,5-bis-(3-hydroxycarbonyl-4-hydroxy)styrylbenzene (BSB) and used this compound to detect amyloid fibrils in autopsy and biopsy samples from patients with localized amyloidosis, such as familial prion disease, and systemic amyloidosis, such as familial amyloidotic polyneuropathy, amyloid A (AA) amyloidosis, light chain (AL) amyloidosis, and dialysis-related amyloidosis. BSB showed reactions in all Congo red-positive and immunoreactive regions of the samples examined in the study, and some amyloid fibrils in the tissues could be detected more precisely with BSB than with the other methods. In the mouse model of AA amyloidosis, injected BSB reacted with amyloid in all regions in the serial sections in which Congo red staining was positive. A highly sensitive 27-MHz quartz crystal microbalance analysis revealed that BSB showed a significant affinity for amyloid fibrils purified from familial amyloidotic polyneuropathy and dialysis-related amyloidosis samples and suppressed formation of transthyretin amyloid in vitro. These results suggest that BSB may become a valuable tool for detection of amyloid deposits in amyloidosis and of the mechanism of amyloid formation.

Original languageEnglish
Pages (from-to)1751-1759
Number of pages9
JournalLaboratory Investigation
Volume83
Issue number12
DOIs
Publication statusPublished - 2003 Dec

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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