TY - JOUR
T1 - A novel stromal cell-dependent B lymphoid stem-like cell line that induces immunoglobulin gene rearrangement
AU - Matsuda, Ken Ichi
AU - Koguma, Masahito
AU - Okuyama, Ryuhei
AU - Nakazawa, Tomoko
AU - Matsuzaki, Yumi
AU - Nakauchi, Hiromitsu
AU - Yanai, Nobuaki
AU - Terasaki, Tetsuya
AU - Obinata, Masuo
PY - 1999
Y1 - 1999
N2 - A stroma-dependent B lymphoid cell line (B31-1) has been established by coculturing sorted stem cells on a novel bone marrow stromal cell line (TBR31-1). B31-1 cells express B220, but do not express other B lymphoid differentiation markers including CD43, heat stable antigen (HSA), or surface immunoglobulin (Ig)M (sIgM), and their Ig heavy chain (IgH) gene loci are germ-line in configuration. The addition of interleukin (IL)-7 or coculture with another stromal cell line, ST2, induces D-J rearrangement of the IgH gene and B lymphocyte differentiation markers. B31-1 cells restore an in vivo repopulation activity to lethally irradiated mice, and the repopulated cells differentiate to HSA+ pre-B cells. Continuous coculture results in two distinct populations, B220- c-Kit+ cells and B220+ c-Kit+ cells; B220- c-Kit+ cells are self-renewed and differentiate to B220+ c-Kit+ cells, while B220+ c-Kit+ cells produce only B220+ c-Kit+ cells. Both B220- and B220+ cells similarly express the IgH germ-line transcript (Iμ), mRNAs for recombinase (TdT, Rag-1, and Rag-2), and lymphoid-specific transcription factors (Pax-5, EBF, E12/E47, Oct-2, and Ikaros), but the DNA binding activity of Pax-5, EBF, Oct-2, and E2A are low in B220- cells and while high in B220+ cells. These results suggest the existence of at least two active states in the IgH locus before the induction of IgH gene rearrangement during B lymphopoietic development.
AB - A stroma-dependent B lymphoid cell line (B31-1) has been established by coculturing sorted stem cells on a novel bone marrow stromal cell line (TBR31-1). B31-1 cells express B220, but do not express other B lymphoid differentiation markers including CD43, heat stable antigen (HSA), or surface immunoglobulin (Ig)M (sIgM), and their Ig heavy chain (IgH) gene loci are germ-line in configuration. The addition of interleukin (IL)-7 or coculture with another stromal cell line, ST2, induces D-J rearrangement of the IgH gene and B lymphocyte differentiation markers. B31-1 cells restore an in vivo repopulation activity to lethally irradiated mice, and the repopulated cells differentiate to HSA+ pre-B cells. Continuous coculture results in two distinct populations, B220- c-Kit+ cells and B220+ c-Kit+ cells; B220- c-Kit+ cells are self-renewed and differentiate to B220+ c-Kit+ cells, while B220+ c-Kit+ cells produce only B220+ c-Kit+ cells. Both B220- and B220+ cells similarly express the IgH germ-line transcript (Iμ), mRNAs for recombinase (TdT, Rag-1, and Rag-2), and lymphoid-specific transcription factors (Pax-5, EBF, E12/E47, Oct-2, and Ikaros), but the DNA binding activity of Pax-5, EBF, Oct-2, and E2A are low in B220- cells and while high in B220+ cells. These results suggest the existence of at least two active states in the IgH locus before the induction of IgH gene rearrangement during B lymphopoietic development.
KW - B-cell differentiation
KW - Early B-cell progenitor
KW - Hematopoietic stem cell
KW - Immunoglobulin gene rearrangement
KW - Stromal cell
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U2 - 10.1093/oxfordjournals.jbchem.a022326
DO - 10.1093/oxfordjournals.jbchem.a022326
M3 - Article
C2 - 10050050
AN - SCOPUS:0032938764
VL - 125
SP - 602
EP - 612
JO - Journal of Biochemistry
JF - Journal of Biochemistry
SN - 0021-924X
IS - 3
ER -