A novel somatic point mutation of the RET proto-oncogene in tumor tissues of small cell lung cancer patients

Hitoyasu Futami; Shinichi Egawa; Toshihiko Tsukada; Kouji Maruyama; Shuji Bandoh; Masayuki Noguchi; Ken Yamaguchi

doi:10.1016/0910-5050(96)85251-3

A novel somatic point mutation of the RET proto-oncogene in tumor tissues of small cell lung cancer patients

Hitoyasu Futami, Shinichi Egawa, Toshihiko Tsukada, Kouji Maruyama, Shuji Bandoh, Masayuki Noguchi, Ken Yamaguchi

Research output: Contribution to journal › Article › peer-review

Abstract

We examined whether the novel point mutation from GCC (Ala) to GAC (Asp) at codon 664 in exon 11 of RET proto-oncogene, which we had found in two small cell lung carcinoma (SCLC) cell lines, existed in genomic DNA of tumor tissues of the two SCLC patients from whom these SCLC cell lines were derived. Sequence analysis revealed that point mutation identical to that of the SCLC cell lines was present in amplified alleles of single-strand conformational variants in genomic DNA of the tumor tissues, whereas it was not detected in genomic DNA of non-tumor tissues of the patients. These results indicate that this mutation had initially occurred in the SCLC patients and was of somatic origin.

Original language	English
Pages (from-to)	1127-1130
Number of pages	4
Journal	Japanese Journal of Cancer Research
Volume	86
Issue number	12
DOIs	https://doi.org/10.1016/0910-5050(96)85251-3
Publication status	Published - 1995 Dec 1
Externally published	Yes

Keywords

Medullary thyroid carcinoma
Multiple endocrine neoplasia
Point mutation
RET
Small cell lung carcinoma

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0910-5050(96)85251-3

Cite this

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title = "A novel somatic point mutation of the RET proto-oncogene in tumor tissues of small cell lung cancer patients",

abstract = "We examined whether the novel point mutation from GCC (Ala) to GAC (Asp) at codon 664 in exon 11 of RET proto-oncogene, which we had found in two small cell lung carcinoma (SCLC) cell lines, existed in genomic DNA of tumor tissues of the two SCLC patients from whom these SCLC cell lines were derived. Sequence analysis revealed that point mutation identical to that of the SCLC cell lines was present in amplified alleles of single-strand conformational variants in genomic DNA of the tumor tissues, whereas it was not detected in genomic DNA of non-tumor tissues of the patients. These results indicate that this mutation had initially occurred in the SCLC patients and was of somatic origin.",

keywords = "Medullary thyroid carcinoma, Multiple endocrine neoplasia, Point mutation, RET, Small cell lung carcinoma",

author = "Hitoyasu Futami and Shinichi Egawa and Toshihiko Tsukada and Kouji Maruyama and Shuji Bandoh and Masayuki Noguchi and Ken Yamaguchi",

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doi = "10.1016/0910-5050(96)85251-3",

language = "English",

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publisher = "Wiley-Blackwell",

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T1 - A novel somatic point mutation of the RET proto-oncogene in tumor tissues of small cell lung cancer patients

AU - Futami, Hitoyasu

AU - Egawa, Shinichi

AU - Tsukada, Toshihiko

AU - Maruyama, Kouji

AU - Bandoh, Shuji

AU - Noguchi, Masayuki

AU - Yamaguchi, Ken

PY - 1995/12/1

Y1 - 1995/12/1

N2 - We examined whether the novel point mutation from GCC (Ala) to GAC (Asp) at codon 664 in exon 11 of RET proto-oncogene, which we had found in two small cell lung carcinoma (SCLC) cell lines, existed in genomic DNA of tumor tissues of the two SCLC patients from whom these SCLC cell lines were derived. Sequence analysis revealed that point mutation identical to that of the SCLC cell lines was present in amplified alleles of single-strand conformational variants in genomic DNA of the tumor tissues, whereas it was not detected in genomic DNA of non-tumor tissues of the patients. These results indicate that this mutation had initially occurred in the SCLC patients and was of somatic origin.

AB - We examined whether the novel point mutation from GCC (Ala) to GAC (Asp) at codon 664 in exon 11 of RET proto-oncogene, which we had found in two small cell lung carcinoma (SCLC) cell lines, existed in genomic DNA of tumor tissues of the two SCLC patients from whom these SCLC cell lines were derived. Sequence analysis revealed that point mutation identical to that of the SCLC cell lines was present in amplified alleles of single-strand conformational variants in genomic DNA of the tumor tissues, whereas it was not detected in genomic DNA of non-tumor tissues of the patients. These results indicate that this mutation had initially occurred in the SCLC patients and was of somatic origin.

KW - Medullary thyroid carcinoma

KW - Multiple endocrine neoplasia

KW - Point mutation

KW - RET

KW - Small cell lung carcinoma

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A novel somatic point mutation of the RET proto-oncogene in tumor tissues of small cell lung cancer patients

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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