A novel reporter rat strain that conditionally expresses the bright red fluorescent protein tdTomato

Hiroyuki Igarashi, Kyo Koizumi, Ryosuke Kaneko, Keiko Ikeda, Ryo Egawa, Yuchio Yanagawa, Shin Ichi Muramatsu, Hiroshi Onimaru, Toru Ishizuka, Hiromu Yawo

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    8 Citations (Scopus)

    Abstract

    Despite the strength of the Cre/loxP recombination system in animal models, its application in rats trails that in mice because of the lack of relevant reporter strains. Here, we generated a floxed STOP tdTomato rat that conditionally expresses a red fluorescent protein variant (tdTomato) in the presence of exogenous Cre recombinase. The tdTomato signal vividly visualizes neurons including their projection fibers and spines without any histological enhancement. In addition, a transgenic rat line (FLAME) that ubiquitously expresses tdTomato was successfully established by injecting intracytoplasmic Cre mRNA into fertilized ova. Our rat reporter system will facilitate connectome studies as well as the visualization of the fine structures of genetically identified cells for long periods both in vivo and ex vivo. Furthermore, FLAME is an ideal model for organ transplantation research owing to improved traceability of cells/tissues.

    Original languageEnglish
    Article numbere0155687
    JournalPloS one
    Volume11
    Issue number5
    DOIs
    Publication statusPublished - 2016 May 1

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Agricultural and Biological Sciences(all)
    • General

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  • Cite this

    Igarashi, H., Koizumi, K., Kaneko, R., Ikeda, K., Egawa, R., Yanagawa, Y., Muramatsu, S. I., Onimaru, H., Ishizuka, T., & Yawo, H. (2016). A novel reporter rat strain that conditionally expresses the bright red fluorescent protein tdTomato. PloS one, 11(5), [e0155687]. https://doi.org/10.1371/journal.pone.0155687