A novel reporter mouse strain that expresses enhanced green fluorescent protein upon Cre-mediated recombination

Shunsuke Kawamoto, Hitoshi Niwa, Fumi Tashiro, Shigetoshi Sano, Gen Kondoh, Junji Takeda, Koichi Tabayashi, Jun Ichi Miyazaki

Research output: Contribution to journalArticle

259 Citations (Scopus)

Abstract

The success of Cre-mediated conditional gene targeting depends on the specificity of Cre recombinase expression in Cre-transgenic mouse lines. As a tool to evaluate the specificity of Cre expression, we developed a reporter transgenic mouse strain that expresses enhanced green fluorescent protein (EGFP) upon Cre-mediated recombination. We demonstrate that the progeny resulting from a cross between this reporter strain and a transgenic strain expressing Cre in zygotes show ubiquitous EGFP fluorescence. This reporter strain should be useful to monitor the Cre expression directed by various promoters in transgenic mice, including mice in which Cre is expressed transiently during embryogenesis under a developmentally regulated promoter. Copyright (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)263-268
Number of pages6
JournalFEBS Letters
Volume470
Issue number3
DOIs
Publication statusPublished - 2000 Mar 31

Keywords

  • Cre/loxP recombination
  • Green fluorescent protein
  • Keratin 5 promoter
  • Transgenic mouse
  • β-Actin promoter

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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  • Cite this

    Kawamoto, S., Niwa, H., Tashiro, F., Sano, S., Kondoh, G., Takeda, J., Tabayashi, K., & Miyazaki, J. I. (2000). A novel reporter mouse strain that expresses enhanced green fluorescent protein upon Cre-mediated recombination. FEBS Letters, 470(3), 263-268. https://doi.org/10.1016/S0014-5793(00)01338-7