A novel protein phosphatase 2C family member (PP2Cζ) is able to associate with ubiquitin conjugating enzyme 9 1

Mitsuhiro Kashiwaba, Koji Katsura, Motoko Ohnishi, Mutsuo Sasaki, Hiromitsu Tanaka, Yoshitake Nishimune, Takayasu Kobayashi, Shinri Tamura

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

In this study we have cloned a novel member of mouse protein phosphatase 2C family, PP2Cζ, which is composed of 507 amino acids and has a unique N-terminal region. The overall similarity of the amino acid sequence between PP2Cζ and PP2Cα was 22%. On Northern blot analysis PP2Cζ was found to be expressed specifically in the testicular germ cells. PP2Cζ expressed in COS7 cells was able to associate with ubiquitin conjugating enzyme 9 (UBC9) and the association was enhanced by co-expression of small ubiquitin-related modifier-1 (SUMO-1), suggesting that PP2Cζ exhibits its specific role through its SUMO-induced recruitment to UBC9.

Original languageEnglish
Pages (from-to)197-202
Number of pages6
JournalFEBS Letters
Volume538
Issue number1-3
DOIs
Publication statusPublished - 2003 Mar 13

Keywords

  • Protein phosphatase 2C
  • Small ubiquitin-related modifier-1
  • Testicular germ cell
  • Ubiquitin conjugating enzyme 9
  • Yeast two-hybrid screening

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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    Kashiwaba, M., Katsura, K., Ohnishi, M., Sasaki, M., Tanaka, H., Nishimune, Y., Kobayashi, T., & Tamura, S. (2003). A novel protein phosphatase 2C family member (PP2Cζ) is able to associate with ubiquitin conjugating enzyme 9 1. FEBS Letters, 538(1-3), 197-202. https://doi.org/10.1016/S0014-5793(03)00153-4