A novel orthotopic mouse model of lung metastasis using fluorescent patient-derived osteosarcoma cells

Hiromichi Oshiro, Yasunori Tome, Kentaro Miyake, Takashi Higuchi, Norihiko Sugisawa, Jun Ho Park, Zhiying Zhang, Sahar Razmjooei, Fuminori Kanaya, Kotaro Nishida, Robert M. Hoffman

Research output: Contribution to journalArticlepeer-review

Abstract

Background: A mouse model of metastatic osteosarcoma is imperative to identify effective agents for metastatic osteosarcoma, which is a recalcitrant disease. In the present study, we established osteosarcoma patient-derived cells (OS-PDCs) and transfected them with green fluorescent protein (GFP). Materials and Methods: The OS-PDCs were transfected with GFP-lentivirus. GFP-expressing OS-PDCs (2.0×105) were then injected into the tibia of nude mice to establish the patient-derived orthotopic cell (PDOC) model (n=3). Six weeks after injection, the primary tumor and each organ were resected and imaged. Results: Primary orthotopic tumors were established in two out of three mice. The GFPexpressing OS-PDCs in the PDOC model were visualized. Multiple GFP-expressing lung metastases were detected in one of the two mice with primary tumor. Conclusion: The present study proves the concept that a GFP-expressing PDOC model can mimic clinical lung-metastatic osteosarcoma. This model can serve as a paradigm to screen for effective drugs for osteosarcoma lung metastasis.

Original languageEnglish
Pages (from-to)635-640
Number of pages6
JournalAnticancer research
Volume41
Issue number2
DOIs
Publication statusPublished - 2021 Feb
Externally publishedYes

Keywords

  • Green fluorescent protein
  • Imaging
  • Lung metastasis
  • Metastasis
  • Osteosarcoma
  • PDC
  • PDOX
  • Patient-derived cells
  • Patient-derived orthotopic xenograft

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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