A novel non-thermostable deuterolysin from Aspergillus oryzae

Hiroshi Maeda, Toru Katase, Daisuke Sakai, Michio Takeuchi, Ken Ichi Kusumoto, Hitoshi Amano, Hiroki Ishida, Keietsu Abe, Youhei Yamagata

Research output: Contribution to journalArticlepeer-review

Abstract

Three putative deuterolysin (EC 3.4.24.29) genes (deuA, deuB, and deuC) were found in the Aspergillus oryzae genome database (http://www.bio.nite.go.jp/dogan/project/view/AO). One of these genes, deuA, was corresponding to NpII gene, previously reported. DeuA and DeuB were overexpressed by recombinant A. oryzae and were purified. The degradation profiles against protein substrates of both enzymes were similar, but DeuB showed wider substrate specificity against peptidyl MCA-substrates compared with DeuA. Enzymatic profiles of DeuB except for thermostability also resembled those of DeuA. DeuB was inactivated by heat treatment above 80° C, different from thermostable DeuA. Transcription analysis in wild type A. oryzae showed only deuB was expressed in liquid culture, and the addition of the proteinous substrate upregulated the transcription. Furthermore, the NaNO3 addition seems to eliminate the effect of proteinous substrate for the transcription of deuB.

Original languageEnglish
Pages (from-to)1813-1819
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume80
Issue number9
DOIs
Publication statusPublished - 2016 Jan 1

Keywords

  • Aspergillus oryzae
  • Deuterolysin
  • Expression specificity
  • Thermostability

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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