TY - JOUR
T1 - A novel method of vesicle preparation by simple dilution of bicelle solution
AU - Taguchi, Shogo
AU - Kang, Bong Su
AU - Suga, Keishi
AU - Okamoto, Yukihiro
AU - Jung, Ho Sup
AU - Umakoshi, Hiroshi
N1 - Funding Information:
The author thanks Professor Takuji Yamamoto for his assistance with the DLS measurement. This work was primarily supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI Grant-in-Aids for Scientific Research (A) (26249116). The author (S. T.) express his gratitude for Sasakawa Scientific Research Grant from the Japan Science Society, JSS (29-218) and for the Special Research Grant from the University of Hyogo . The author (H.-S. J.) express his gratitude for JSPS Invitational Fellowships for Research in Japan (L18525).
Funding Information:
The author thanks Professor Takuji Yamamoto for his assistance with the DLS measurement. This work was primarily supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI Grant-in-Aids for Scientific Research (A) (26249116). The author (S. T.) express his gratitude for Sasakawa Scientific Research Grant from the Japan Science Society, JSS (29-218) and for the Special Research Grant from the University of Hyogo. The author (H.-S. J.) express his gratitude for JSPS Invitational Fellowships for Research in Japan (L18525).
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/10/15
Y1 - 2020/10/15
N2 - We prepared phospholipid vesicles in continuous dilution from small phospholipid bilayer bicelles. As vesicles are useful as delivery carriers, it is necessary to develop a method of continuous encapsulation. Bicelles are composed of long-chain 1,2-dimyristoyl-sn-glycero-3-phosphocholine in bilayers and short-chain 1,2-dihexanoyl-sn-glycero-3-phosphocholine as a detergent. Bicelles were diluted in a batch system and a flow system and their membrane properties were compared; sizes were analyzed by dynamic light scattering and membrane properties by fluorescence spectroscopy. In the flow system, the initial bicelles (10−20 nm) coalesced by dilution and hydration of the detergent into large vesicles (>30 nm). Uptake of methylene blue solution revealed the internal aqueous phase. Transmission electron microscopy confirmed the formation of vesicles by dilution. The membrane fluidity of the vesicles showed an ordered gel phase. The results suggest that bicelles can form vesicles by continuous dilution. This method is suitable for encapsulating materials.
AB - We prepared phospholipid vesicles in continuous dilution from small phospholipid bilayer bicelles. As vesicles are useful as delivery carriers, it is necessary to develop a method of continuous encapsulation. Bicelles are composed of long-chain 1,2-dimyristoyl-sn-glycero-3-phosphocholine in bilayers and short-chain 1,2-dihexanoyl-sn-glycero-3-phosphocholine as a detergent. Bicelles were diluted in a batch system and a flow system and their membrane properties were compared; sizes were analyzed by dynamic light scattering and membrane properties by fluorescence spectroscopy. In the flow system, the initial bicelles (10−20 nm) coalesced by dilution and hydration of the detergent into large vesicles (>30 nm). Uptake of methylene blue solution revealed the internal aqueous phase. Transmission electron microscopy confirmed the formation of vesicles by dilution. The membrane fluidity of the vesicles showed an ordered gel phase. The results suggest that bicelles can form vesicles by continuous dilution. This method is suitable for encapsulating materials.
KW - Bicelle
KW - Continuous vesicle preparation
KW - Dilution process
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U2 - 10.1016/j.bej.2020.107725
DO - 10.1016/j.bej.2020.107725
M3 - Article
AN - SCOPUS:85088796519
VL - 162
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
SN - 1369-703X
M1 - 107725
ER -