A novel method for the detection of protease and the development of extracellular protease in early growth stages of Lactobacillus delbrueckii ssp. bulgaricus

Yasushi Kawai, Kiyoshi Tadokoro, Ryotaro Konomi, Kazumi Itoh, Tadao Saito, Haruki Kitazawa, Takatoshi Itoh

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A novel method of rapid detection using microplate wells with casein and calcium ions was developed for measuring protease activity. This new method is based on the theory that the hydrolysis by the proteases reduces the turbidity of the solution without coagulation. The three Lactobacillus delbrueckii ssp. bulgaricus strains that were tested secreted a protease with activity that reached its maximum 4 to 8 h after incubation (log phase) and then decreased rapidly. The new method allows the protease activity to be detected and measured more rapidly, easily, and economically than do other methods with fluorescent reagents or radioisotopes, and the new method is especially useful for analyzing large numbers of test samples (e.g., chromatographically fractionated eluates).

Original languageEnglish
Pages (from-to)481-485
Number of pages5
JournalJournal of Dairy Science
Volume82
Issue number3
DOIs
Publication statusPublished - 1999 Mar

Keywords

  • Extracellular protease
  • Lactic acid bacteria
  • Lactobacillus delbrueckii ssp. bulgaricus
  • Microplate

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

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