TY - JOUR
T1 - A novel method for detection of endo-xyloglucan transferase
AU - Nishitani, Kazuhiko
N1 - Funding Information:
The author wish to thank Professor Susumu Hizukuri and Dr. Yasuhito Takeda (Kagoshima University) for providing pullulans. This work was supported by a Grant-in-Aid for Scientific Research (no. 03804055) from the Ministry of Education, Science and Culture, Japan.
PY - 1992/12
Y1 - 1992/12
N2 - A new approach has been developed for quantification of the activity of endo-xyloglucan transferase, a novel enzyme that mediates the transfer of a segment of one xyloglucan molecule to another xyloglucan molecule. Purified xyloglucans with defined molecular-weight distributions and their fluorescent derivatives (pyridylamino xyloglucans) were used as substrates for the enzymatic reaction. The transferase activity was quantified by monitoring changes in molecular-weight distributions of substrates by an alkali compatible gel permeation chromatographic system, equipped with a pulsed amperometric detector and a fluorescence detector. This new method was applied to the rapid detection and characterization of a novel transferase derived from plant tissues.
AB - A new approach has been developed for quantification of the activity of endo-xyloglucan transferase, a novel enzyme that mediates the transfer of a segment of one xyloglucan molecule to another xyloglucan molecule. Purified xyloglucans with defined molecular-weight distributions and their fluorescent derivatives (pyridylamino xyloglucans) were used as substrates for the enzymatic reaction. The transferase activity was quantified by monitoring changes in molecular-weight distributions of substrates by an alkali compatible gel permeation chromatographic system, equipped with a pulsed amperometric detector and a fluorescence detector. This new method was applied to the rapid detection and characterization of a novel transferase derived from plant tissues.
KW - Cell wall
KW - Endo-xyloglucan transferase
KW - Pulsed amperometry detector
KW - Pyridylamino xyloglucan
KW - Vigna angularis Ohwi et Ohashi (azuki bean)
KW - Xyloglucan
UR - http://www.scopus.com/inward/record.url?scp=0002732586&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0002732586&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0002732586
VL - 33
SP - 1159
EP - 1164
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 8
ER -