A novel method for detection of endo-xyloglucan transferase

Kazuhiko Nishitani

    Research output: Contribution to journalArticlepeer-review

    27 Citations (Scopus)

    Abstract

    A new approach has been developed for quantification of the activity of endo-xyloglucan transferase, a novel enzyme that mediates the transfer of a segment of one xyloglucan molecule to another xyloglucan molecule. Purified xyloglucans with defined molecular-weight distributions and their fluorescent derivatives (pyridylamino xyloglucans) were used as substrates for the enzymatic reaction. The transferase activity was quantified by monitoring changes in molecular-weight distributions of substrates by an alkali compatible gel permeation chromatographic system, equipped with a pulsed amperometric detector and a fluorescence detector. This new method was applied to the rapid detection and characterization of a novel transferase derived from plant tissues.

    Original languageEnglish
    Pages (from-to)1159-1164
    Number of pages6
    JournalPlant and Cell Physiology
    Volume33
    Issue number8
    Publication statusPublished - 1992 Dec

    Keywords

    • Cell wall
    • Endo-xyloglucan transferase
    • Pulsed amperometry detector
    • Pyridylamino xyloglucan
    • Vigna angularis Ohwi et Ohashi (azuki bean)
    • Xyloglucan

    ASJC Scopus subject areas

    • Physiology
    • Plant Science
    • Cell Biology

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