A novel method for detection of endo-xyloglucan transferase

Kazuhiko Nishitani

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

A new approach has been developed for quantification of the activity of endo-xyloglucan transferase, a novel enzyme that mediates the transfer of a segment of one xyloglucan molecule to another xyloglucan molecule. Purified xyloglucans with defined molecular-weight distributions and their fluorescent derivatives (pyridylamino xyloglucans) were used as substrates for the enzymatic reaction. The transferase activity was quantified by monitoring changes in molecular-weight distributions of substrates by an alkali compatible gel permeation chromatographic system, equipped with a pulsed amperometric detector and a fluorescence detector. This new method was applied to the rapid detection and characterization of a novel transferase derived from plant tissues.

Original languageEnglish
Pages (from-to)1159-1164
Number of pages6
JournalPlant and Cell Physiology
Volume33
Issue number8
Publication statusPublished - 1992 Dec
Externally publishedYes

Keywords

  • Cell wall
  • Endo-xyloglucan transferase
  • Pulsed amperometry detector
  • Pyridylamino xyloglucan
  • Vigna angularis Ohwi et Ohashi (azuki bean)
  • Xyloglucan

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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