A method was proposed to provide dosimeters of exposure to lipid hydroxyperoxide modification to arginine containing peptides. The 4-Oxo-2-nonenal was reacted with amino acids, peptides and proteins in transition metal ion free 100 mM MOPS buffer. The protein samples were reacted with dithiothreitol and iodoacetoamide for cisteine modification and then catalyzed by trypsin or V8 protease before LC/MS. Results show that no modification occurred on the N-terminus as b 2-NH 3 and b 3-NH 3 were observed.
|Number of pages||2|
|Publication status||Published - 2002 Dec 1|
|Event||Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States|
Duration: 2002 Jun 2 → 2002 Jun 6
|Other||Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics|
|Period||02/6/2 → 02/6/6|
ASJC Scopus subject areas