A novel lectin from sarcophaga: Its purification, characterization, and cDNA cloning

Yoshifumi Fujita, Shoichiro Kurata, Ko Ichi Homma, Shunji Natori

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A novel C-type lectin that agglutinates rabbit red cells was purified from NIH-Sape-4 cells derived from the flesh fly (Sarcophaga peregrina), and its cDNA was isolated. This lectin, named granulocytin, appeared to be a trimer of a 20-kDa subunit consisting of 151 amino acid residues. The gene for granulocytin was activated in third instar larvae, and its expression was enhanced when the larval body wall was injured. In third instar larvae, granulocytin was found to be synthesized by hemocytes and secreted into the hemolymph. The molecular mass and gene expression patterns of granulocytin were very similar to those of Drosophila lectin that we reported previously (Haq, S., Kubo, T., Kurata, S., Kobayashi, A., and Natori, S. (1996) J. Biol. Chem. 271, 2021320218). However, these two lectins showed amine acid identifies of 20% at most, and no significant hapten sugar for granulocytin was identified.

Original languageEnglish
Pages (from-to)9667-9672
Number of pages6
JournalJournal of Biological Chemistry
Volume273
Issue number16
DOIs
Publication statusPublished - 1998 Apr 17
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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