A novel imprinted gene, HYMAI, is located within an imprinted domain on human chromosome 6 containing ZAC

Takahiro Arima, Robert A. Drewell, Mitsuo Oshimura, Norio Wake, M. Azim Surani

Research output: Contribution to journalArticlepeer-review

62 Citations (Scopus)

Abstract

Transient neonatal diabetes mellitus (TNDM) is a rare disease characterized by intrauterine growth retardation, dehydration, and failure to thrive due to a lack of normal insulin secretion. This disease is associated with paternal uniparental disomy or paternal duplication of chromosome 6, suggesting that the causative gene(s) for TNDM is imprinted. Recently, Gardner et al. (1999, J. Med. Genet. 36: 192-196) proposed that a candidate gene for TNDM lies within chromosome 6q24.1-q24.3. To find human imprinted genes, we performed a database search for EST sequences that mapped to this region, followed by RT-PCR analysis using monochromosomal hybrid cells with a human chromosome 6 of defined parental origin. Here we report the identification of a novel imprinted gene, HYMAI. This gene exhibits differential DNA methylation between the two parental alleles at an adjacent CpG island and is expressed only from the paternal chromosome. A previously characterized imprinted gene, ZAC/LOT1, is located 70 kb downstream of HYMAI and is also expressed only from the paternal allele. In the pancreas, both genes are moderately expressed. HYMAI and ZAC/LOT1 are therefore candidate genes involved in TNDM. Furthermore, the human chromosome 6q24 region is syntenic to mouse chromosome 10 and represents a novel imprinted domain. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)248-255
Number of pages8
JournalGenomics
Volume67
Issue number3
DOIs
Publication statusPublished - 2000 Aug 1
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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