TY - JOUR
T1 - A novel importin α from rice, a component involved in the process of nuclear protein transport
AU - Iwasaki, Toshisuke
AU - Matsuki, Rikyu
AU - Shoji, Kazuhiro
AU - Sanmiya, Kazutsuka
AU - Miyao, Mitsue
AU - Yamamoto, Naoki
N1 - Funding Information:
We thank Dr. N. Imamoto of Osaka University for providing the BSA-peptide conjugates and GST-mouse importin α. This work was supported by the Special Coordination Fund for Promoting Science and Technology, Enhancement of Center-of-Excellence, to NIAR from the Science and Technology Agency of Japan, and in part by a fund from the Ministry of Agriculture, Forestry and Fisheries of Japan, and a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan.
PY - 1998/5/29
Y1 - 1998/5/29
N2 - In eukaryotes, nuclear proteins that are transported into nuclei have nuclear localization signals (NLSs), which are recognized by proteins called importin α. We isolated a rice cDNA, #61L, and the corresponding gene that encodes a protein, which shows significant homology to the importin α. Although the encoded protein had only 23-27% amino acid identity to the importin as from various organisms including plants, the fusion protein with glutathione S-transferase showed a specific binding activity to the NLS of SV40 T-antigen. These results suggest that the rice #61L protein is a novel importin α in plants.
AB - In eukaryotes, nuclear proteins that are transported into nuclei have nuclear localization signals (NLSs), which are recognized by proteins called importin α. We isolated a rice cDNA, #61L, and the corresponding gene that encodes a protein, which shows significant homology to the importin α. Although the encoded protein had only 23-27% amino acid identity to the importin as from various organisms including plants, the fusion protein with glutathione S-transferase showed a specific binding activity to the NLS of SV40 T-antigen. These results suggest that the rice #61L protein is a novel importin α in plants.
KW - Importin α
KW - Nuclear localization signal
KW - Nuclear protein import
KW - Rice (Oryza sativa)
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U2 - 10.1016/S0014-5793(98)00540-7
DO - 10.1016/S0014-5793(98)00540-7
M3 - Article
C2 - 9654145
AN - SCOPUS:0032577530
VL - 428
SP - 259
EP - 262
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 3
ER -