A novel importin α from rice, a component involved in the process of nuclear protein transport

Toshisuke Iwasaki; Rikyu Matsuki; Kazuhiro Shoji; Kazutsuka Sanmiya; Mitsue Miyao; Naoki Yamamoto

doi:10.1016/S0014-5793(98)00540-7

A novel importin α from rice, a component involved in the process of nuclear protein transport

Toshisuke Iwasaki, Rikyu Matsuki, Kazuhiro Shoji, Kazutsuka Sanmiya, Mitsue Miyao, Naoki Yamamoto

Research output: Contribution to journal › Article

7 Citations (Scopus)

Abstract

In eukaryotes, nuclear proteins that are transported into nuclei have nuclear localization signals (NLSs), which are recognized by proteins called importin α. We isolated a rice cDNA, #61L, and the corresponding gene that encodes a protein, which shows significant homology to the importin α. Although the encoded protein had only 23-27% amino acid identity to the importin as from various organisms including plants, the fusion protein with glutathione S-transferase showed a specific binding activity to the NLS of SV40 T-antigen. These results suggest that the rice #61L protein is a novel importin α in plants.

Original language	English
Pages (from-to)	259-262
Number of pages	4
Journal	FEBS Letters
Volume	428
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(98)00540-7
Publication status	Published - 1998 May 29
Externally published	Yes

Keywords

Importin α
Nuclear localization signal
Nuclear protein import
Rice (Oryza sativa)

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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Iwasaki, T., Matsuki, R., Shoji, K., Sanmiya, K., Miyao, M., & Yamamoto, N. (1998). A novel importin α from rice, a component involved in the process of nuclear protein transport. FEBS Letters, 428(3), 259-262. https://doi.org/10.1016/S0014-5793(98)00540-7

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AU - Miyao, Mitsue

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